褪黑素对H22肝癌小鼠的免疫调节及抗肿瘤作用

Experimental study of immunomodulation and antitumor effects of melatonin in H22 hepatoma-bearing mice

目的：观察褪黑素（ＭＬＴ）对荷瘤小鼠的免疫调节及抗肿瘤作用。方法：以Ｈ２２肝癌小鼠为模型，采用流式细胞仪技术及四甲基偶氮唑盐（ＭＴＴ）比色法测定荷瘤小鼠的各项免疫指标。结果：ＭＬＴ能提高荷瘤小鼠ＣＤ４＋／ＣＤ８＋值，协同ＩＬ－２提高外周血淋巴细胞及嗜酸性粒细胞数量，增强脾细胞ＮＫ及ＬＡＫ活性，促进ＩＬ－２的诱生，并且ＭＬＴ能抑制荷瘤小鼠的肿瘤生长，延长其存活时间，与ＩＬ－２存在明显协同性。ＭＬＴ在体外对Ｈ２２细胞无直接影响。结论：ＭＬＴ可作为一种生物反应调节剂（ＢＲＭ）在肿瘤的免疫治疗中发挥作用。

Objective:To observe the immunomodulation and antitumor effects of melatonin (MLT )in tumor-bearing mice. Methods:By the method of flow cytometry and MTT colorimetry,the immunological indexes of the H22 hepatoma-bearing mice were investigated. Results: MLT could increase the CD4+/CD8+cell ratio in the tumor-bearing mouse peripheral blood, cooperate IL-2 to promote the proliferation of lymphocytes and eosinophiles,increase the NK and LAK activity of spleenocytes, and enhance the production of IL-2 induced from the spleenocytes.MLT could also inhibit the tumor growth.prolong the life-span of the tumor-bearing mice in vivo and there was a synergetic action between IL-2 and MLT,while there was no effect on H22 hepatoma cells when MLT was used in vitro.Conclusion:MLT may have great effects on tumor immunotherapy as one of biological reation modulators(BRM).