维生素A对实验性大鼠肝纤维化的影响

Effects of vitamin A on hepatic fibrosis induced by carbon tetrachloride in rats

目的：观察中等剂量维生素Ａ对四氯化碳（ＣＣｌ４）诱导的实验性大鼠肝纤维化的影响。方法：在四氯化碳诱导大鼠肝纤维化的同时，给以１００ｍｇ／ｋｇ体重维生素Ａ皮下注射。检测在早（３周）、中（６周）、晚（１２周）期大鼠肝组织羟脯氨酸含量及血清中透明质酸及谷丙转氨酶水平，并观察肝脏的组织病理改变。结果：治疗组早期血清透明质酸及组织病理学的炎症程度均显著低于ＣＣｌ４对照组（Ｐ＜０．０５），中、晚期肝组织羟脯氨酸含量及晚期肝组织学纤维化程度虽低于ＣＣｌ４对照组，但相差不显著。结论：上述剂量维生素Ａ能减轻ＣＣｌ４所致大鼠的早期肝脏损害，它可能有益于减轻其后的肝纤维化。

Objective: To observe the effects of moderate dose of vitamin A on experimental hepatic fibrosis induced by carbon tetrachloride(CCl4) in rats. Methods:During the administration of CCl4, animals were treated with retinol acetate simultaneously.The amount of hydroxyproline(Hyp)in hepatic tissue and the levels of hyaluronic acid (HA ) and ALT in serum were determined at early(3 weeks),middle (6 weeks) and late (12 weeks)stage separately. Histopathological changes were also observed. Rusults:The HA level was reduced and the histopathological inflammatory change was less severe significantly(p<0. 05) at early stage in the animals treated with retinol acetate, as compared with those received CCl4 only. Hyp content and the histopathological fibrotic change were less but no statistical significance than those received CCl4 alone.Conclusion :Moderate dose of vitamin A may protect the liver cell from damage caused by CCl4 at early stage. It may be beneficial to decrease of the severity of liver fibrosis.