摘要
目的：克隆人降钙素ｃＤＮＡ。方法和结果：应用ＡＢＩ３９１ＤＮＡ合成仪，将人降钙素ｃＤＮＡ分成６个片段合成。退火、磷酸化、连接，最终将人降钙素ｃＤＮＡ克隆于载体ｐＧＥＭ７ｚ（＋），经ＤＮＡ双链测序，证明克隆到的人降钙素ｃＤＮＡ序列与设计的完全一致。结论：本研究为寡肽ｃＤＮＡ（或基因）的化学合成与克隆提供了简捷、可靠的方法；为人的降钙素ｃＤＮＡ的高效表达奠定了基础。
Objective: Chemical synthesis and cloning of human calcitonin (hCT) cDNA. Methods and Results: A cDNA coding for human calcitonin was divided into six fragments,synthesized by ABI 391 DNA synthesis instruments and cloned into pGEM7Z(+) vector following phosphorylation, annealing and ligation of six fragments and the sequence of cloned hCT cDNA was proved to be the same as designed. Conclusion: A reliable and forthright method of chemical synthesis of oligo-peptide cDNA (or gene)and cloning is provided in this study. This paper is a basic work for expression of hCT cDNA.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
1996年第5期447-449,共3页
Academic Journal of Second Military Medical University
关键词
降钙素
CDNA
化学合成
克隆
human calcitonin
cDNA
chemical synthesis
clone
