盐酸奥芬米洛对二硝基氟苯诱导小鼠迟发型变态反应的影响

Effect of Oxfenmino Hydrochloric Acid on Delayed Allergic Reaction in Mouse Induced by Dinitrofluorobenzene

[目的]观察盐酸奥芬米洛对小鼠迟发型变态反应的影响。[方法]取体重为18～22g的ICR小鼠120只，雌雄各半，按体重随机分6组，即空白对照组、模型对照组、盐酸奥芬米洛高（10．0mg／kg）、中（5．0mg／kg）、低（2．5mg／kg）剂量组和阳性对照（CsA 15mg／kg）组，每组20只，各给药组分别给予相应浓度的相应药物，空白与模型对照组给予蒸馏水10ml／kg，每日1次，连续给药1周后，除空白对照组外，每鼠腹部用1％DNFB溶液均匀涂抹进行致敏，致敏后第5天，将1％DNFB溶液均匀涂抹于小鼠右耳进行攻击，空白对照组同样涂耳但未致敏，攻击后24h，测定右耳的肿胀度和小鼠的脾指数和胸腺指数。[结果]不同剂量的盐酸奥芬米洛能不同程度地降低DNFB致敏ICR小鼠耳廓肿胀度、降低脾脏系数和胸腺指数，[结论]盐酸奥芬米洛可抑制DNFB诱导小鼠迟发型变态反应（DTH），具有抑制小鼠的特异性细胞免疫功能的作用。

[Objective]To determine the effect of Oxfenmino hydrochloric acid on delayed allergic reaction in mouse. [ Method ] 120 ICR mice weighing 18-22g, half female and half male, were randomly and averagely divided into 6 groups according to weight： control group and model group （treated with distilled water at a dosage of 10ml/kg）, three Oxfenmino hydrochloric acid groups （treated with Oxfenmino hydrochloric acid at a dosage of 10.0mg/kg, 5.0mg/kg and 2.5mg/kg respectively） and positive drug group （treated with CsA at a dosage of 15mg/kg） ; they were orally administered one time every day. After a continuous week of administration, all the groups were smeared evenly with 1 % DNFB solution on the abdomen for allergy except the control group. Five days after allergy, 1 % DNFB solution was smeared to right ear of all mice to stimulate the allergic reaction except that the control group was smeared with non-allergenic substance. 24 hours after attack, the auricle swelling, spleen index and thymus index in right mouse were determined. [ Result] Different dosages of Oxfenmino hydrochloric acid benefited to reduce auricle swelling, spleen index and thymus index in mouse with allergic reaction induced by DNFB at different degree. [ Conclusion] Oxfenmino hydrochloric acid can inhibit the delayed allergic reaction in mouse induced by DNFB and depress the ability of specific cell-mediated immunity.