毛细管电泳-间接化学发光法检测染发剂中苯二酚异构体和苯酚

Separation and Determination of Benzenediol Isomers and Phenol in Hair Dye by Capillary Zone Electrophoresis-Chemiluminescence Method

根据某些酚类化合物在碱性条件下具有能猝灭鲁米诺-铁氰化钾体系化学发光信号的特性，建立了毛细管电泳-间接化学发光分离检测苯二酚异构体和苯酚的新方法。电泳缓冲溶液选用7．5mmol／L Na2B4O7-2．5mmol／L Na2HPO4。在优化的化学发光和电泳条件下，对苯二酚、间苯二酚、邻苯二酚和苯酚在10min内可直接实现分离，其检出限（S／N=3）分别为2．9×10^-8mol／L、3．7×10^-7mol／L、8．4×10^-8mol／L和4．4×10^-6mol／L；相对标准偏差（RSD）为2．5％～4．8％（n=5）。通过对不同染发剂实际样品进行分离分析研究，证明该方法可以用于实际染发剂样品的分析测定，结果令人满意。

A capillary electrophoresis （CE） with onQline inhibited chemiluminescence （CL） detection was used for the simultaneous analysis of benzenediol isomers and phenol. It was based on the quenching effect of benzenediol isomers and phenol on the chemiluminescence reaction of luminol with potassium ferricyanide in sodium hydroxide medium. The running buffer was composed of 7.5 mmol/L Na2B4O7-2.5 mmol/L Na2HPO4. Under the optimum conditions, the four phenols were baseline separated and detected in less than 10 min. The detection limits （S/N = 3） for hydroquinone, resorcinol, catechol and phenol were 2.9×10^-8 mol/L, 3.7×10^-7 mol/L, 8.4×10^-8 mol/L and 4.4×10^-6 mol/L respectively. The RSDs were between 2.5% and 4.8% （n = 5）. Finally, the presented method has been successfully applied to real hair dye samples.