荧光分析法测定银杏酚酸

Determination of Total Ginkgolic Acids by Fluorescence Spectrophotometry

根据银杏酚酸在紫外光激发下能产生荧光的性质，建立了检测银杏酚酸的荧光分光光度法。以80％乙醇80℃回流8h提取银杏外种皮，提取液经硅胶柱层析后得酚酸样品。以白果酸为标样，激发波长345nm、发射波长420nm测定银杏酚酸。线性回归方程为，y=2．5024x＋0．4152；线性范围为0．50～100mg／L，相关系数r=0．9922，相对标准偏差2．6％；检出限0．11mg／L。

A fluorescence spectrophotometry for determination was extracted with 80% ethanol by continuous thermal reflux of ginkgolic acids was established. The sample from Exopleura of Ginkgo biloba at 80℃ for 8 hours and the extract was purified by silica gel column chromatography. Total ginkgolic acids was determined by spectrofluorometry with ginkgolic acid as standard reference at λex = 345 nm and λem = 420 nm. The linearity relationship is y = 2.5024x ＋ 0.4152, with correlative coefficient of 0.9922, relative standard deviation （RSD） of 2.6% and detection limit of 0.114 mg/L. Influences of pH and the dissolved impurities were discussed. The results obtained by this method agreed with those by the high performance liquid chromatography with fluorescence detection（FLD）.