血管内皮生长因子D在胃癌中表达的意义

Expression and significance of vascular endothelial growth factor D in gastric cancer

目的研究血管内皮生长因子D(VEGF-D)在胃癌组织内表达的意义。方法选择手术后的100例胃癌(区域淋巴结转移阳性和阴性病例各50例),30例正常胃黏膜设为对照组。利用免疫组化EnVision法,检测研究对象胃组织内VEGF-D和VEGF-C的表达。分析VEGF-D阳性表达与临床病理参数和VEGF-C阳性表达的关系。结果VEGF-C,VEGF-D的阳性染色为棕黄色,主要位于肿瘤细胞的胞质,局灶性或弥散性分布。VEGF-C,VEGF-D阳性表达以(+)或(++)为主;胃癌VEGF-C、VEGF-D阳性表达率分别为51%和60%;对照组分别为10%和20%(P<0.05);VEGF-D阳性高表达(++)与区域淋巴结转移、淋巴管受侵和VEGF-C阳性表达相关,与肿瘤最大径、肿瘤位置、肿瘤浸润深度、肿瘤肉眼分型、肿瘤组织分化、肿瘤血管受侵无关。结论VEGF-D在胃癌淋巴道转移过程中具有较重要的作用。

Objective To clarify expression of vascular endothelial growth factor D（VEGF-D） and its relationship with lymph node metastasis in gastric carcinoma. Methods The subjects were 100 patients with gastric carcinoma （50 cases with positive or negative for lymph node metastasis, respectively） , meanwhile, 30 cases of normal gastric tissues were used as control. Simultaneously, the expression of VEGF-C and D protein in gastric carcinoma tissues and in normal gastric tissues was detected by immunohistochemistry. Results VEGF-C and D can be stained in cytoplasm in gastric carcinoma tissues and normal gastric tissue by immunohistochemistry. VEGF-C and D protein were expressed in 51% （51/100） , 60 %（60/100） of patients, respectively. The VEGF-C and D positive rates in gastric carcinoma were much higher than those of control（P 〈 0.05）. There was a significant correlation between the positive expression （＋＋） of VEGF-D and lymph node metastasis, lymphatic invasion , positive expression of VEGF-C （P 〈0.025）. Conclusion The expression（＋＋） of VEGF-D is closely related to lymph node metastasis in gastric carcinoma.