重组人干扰素α2a凝胶对单纯疱疹病毒感染的疗效

Curative Effect of Recombinant Human Interferon α2a Gel on Herpes Simplex Virus Infection in Animals

目的研究重组人干扰素α2a(rhIFNα2a)凝胶对动物体内单纯疱疹病毒(HSV)感染的疗效。方法建立HSV-1型豚鼠皮肤感染模型和HSV-2型小鼠阴道炎模型,考察rhIFNα2a凝胶抗HSV作用。将模型豚鼠与小鼠各自分为3个试验组,分别用3×105、2×105和1×105IU/g的rhIFNα2a凝胶治疗,并设平行对照(阿昔洛韦)与空白对照(未治疗)组。评价各组的疗效。结果与空白对照组比较,rhIFNα2a凝胶2×105和3×105IU/g剂量组可明显减少豚鼠皮肤组织中病毒含量,降低小鼠的死亡率,延长生存期。rhIFNα2a凝胶与阿昔洛韦的疗效差异无显著意义。结论rhIFNα2a凝胶具有抑制HSV-1致皮肤病变作用,可缩短病程,对HSV-2所致小鼠阴道炎有较好疗效。

Objective To study the curative effect of recombinant human interferon α2a（rhIFNα2a） gel on in vivo infection with herpes simplex virus（HSV） in animals. Methods The guinea pig model of skin infection with HSV-1 and the mouse model of vaginitis caused by HSV-2 infection were established. Divide the model guinea pigs and mice into three test,one parallel control and one blank control groups separately. Treat the animals in the three test groups with rhIFNα2a gel at dosages of 3 × 10^5,2 × 10^5 and 1 × 10^5 IU/g respectively,while those in parallel control groups with acyclovir. However,the animals in blank control groups were untreated. Evaluate the curative effect of each group. Results The rhIFNα2a gel at dosages of 2 × 10^5 and 3 × 10^5 IU/g decreased the HSV-1 content in skin tissue of guinea pigs as well as the mortality rate of mice, and prolonged the survival period of mice significantly. The curative effect of rhIFNα2a gel showed no significant difference with that of acyclovir. Conclusion rhlFNα2a gel inhibited the pathological change on skin caused by HSV-1 and shortened the course of disease. It showed good curative effect on vaginitis caused by HSV- 2 in mice.