组织灌流与贴壁法体外培养奶牛乳腺组织的比较研究

Comparative Study on in vitro Stationary Culture System or Superfusion Culture System of Cow Mammary Tissue

采用组织灌流和组织块贴壁法体外培养奶牛乳腺组织,通过LDH活力测定、台盼蓝染色、琼脂糖凝胶电泳、透射电镜技术比较两种培养方法对奶牛乳腺组织活性及组织超微结构的影响,建立奶牛乳腺组织的培养体系。结果表明,以1mL/h的速度灌流培养的奶牛乳腺组织在DMEM+10%小牛血清培养基中12～60h内保持良好的组织活性和超微结构,贴壁培养的奶牛乳腺组织在DMEM+10%小牛血清的培养基中60～108h内保持良好的组织活性和超微结构,两种培养方法各有优缺点。

Dairy cow mammary tissue was cultured in superfusion system or stationary system, and the influence of these two methods in the activity and uhrastructure of tissue was investigated according to LDH vigor, trypan blue dying, agrose gel electrophoresis, transmission microscope observation. The results showed that the mammary tissue cultured in superfusion system could keep normal tissue activity and ultrastructure within 12 -60h in DMEM plus 10% calf serum, while mammary tissue stationary culture could keep normal tissue activity and ultrastructure within 60 - 108h. Both culture systems had some advantages and disadvantages.