利用小麦微卫星引物建立簇毛麦染色体组特异性标记

Development of Dasypyrum Genome Specific Marker by Using Wheat Microsatellites

选位于普通小麦1A-7A、1B-7B、1D-7D染色体上的102对微卫星引物对多年生簇毛麦、二倍体簇毛麦、小麦-簇毛麦双二倍体与后代和普通小麦中国春、R25、R111、MY11进行了PCR扩增,发现引物对Xgwm301可以在含簇毛麦染色体的材料中扩出一条长415bp的特异片段(命名为Xgwm301/415),而所有供试小麦均未扩出此片段。进而用一套中国春-二倍体簇毛麦附加系来进行扩增,发现1V-7V染色体均可以扩出该片段,说明该片段为簇毛麦1V-7V染色体所共有。因此,Xgwm301/415是簇毛麦染色体组上的一个特异片段,可以用来快速跟踪检测导入到普通小麦背景中的簇毛麦染色体。

One hundred and two SSR primer pairs, distributed in chromosome 1A to 7A, 1B to 7B, 1D to 7D of Triticum aestivum, were investigated on Dasypyrum breviaristatum, D.villosum, wheat-Dasypyrum amphiploids and its derivatives, with the control of common wheat Chinese Spring and elite wheat cultivars. A specific polymorphic DNA fragment of about 400 bp （ 415 bp-long by sequenced, named Xgwm301/415） amplified by primer pair Xgwm301 was obtained in all lines containing Dasypyrum chromosomes, but there were not the case in the tested common wheat. Furthermore, PCR analysis was performed on a set of T. aestivum-Dasypyrum addition, the result showed that all the seven pairs of villosum chromosomes contain Xgwm301/415. Therefore, Xgwm301/415 is a genome -specific polymorphic DNA segment for genera of Dasypyrum, and it could be used as a molecular marker for detection of chromosomes of Dasypyrum in wheat.