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利用椭圆小球藻硝酸还原酶缺失突变体为生物反应器表达兔防御素NP-1蛋白 被引量：4

Expression of Rabbit Neutrophile Peptide-1 in Nitrate Reductase-deficient Mutant of Chlorella ellipsoidea
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摘要 利用转基因小球藻为生物反应器生产兔防御素NP-1蛋白具有重要的应用价值。本研究利用椭圆小球藻(Chlorellaellipsoidea)硝酸还原酶(nitratereductase)缺失突变体为受体,构建了包含NPTII基因和硝酸还原酶基因两个筛选标记的兔防御素蛋白表达载体,采用电激法将目的基因转入椭圆小球藻硝酸还原酶缺失突变体nrm-4,获得了正确表达防御素蛋白的转基因藻,从而表明通过硝酸还原酶作为筛选标记基因并结合硝酸还原酶缺失突变体可作为较好的小球藻生物反应器生产模式。 Application of transgenic Cholrella as bioreactor to express rabbit neutrophile pepetide-1 （NP-1） shows great practical value. In this paper, an NP-1 expression vector containing two selective marker genes NPTII and nitrate reductase gene was constructed. The NP-1 gene was transformed into the nitrate reductase-deficient mutant nrm-4 of Chlorella ellipsoiclea via electroporation, and the transgenic alga expressed the active NP-1 were obtained.
作者 张小宇 王鹏 赵世民 李霞 沈昕 孙勇如 储成才 王义琴
机构地区 中国科学院遗传与发育生物学研究所 北京林业大学生物科学与技术学院
出处 《遗传》 CAS CSCD 北大核心 2006年第12期1580-1584,共5页 Hereditas（Beijing)
基金 国家十五海洋863青年基金项目(编号:2004AA628010)~~
关键词 椭圆小球藻 硝酸还原酶缺失突变体 兔防御素 Chlorella ellipsoidea nitrate reductase-deficient mutant rabbit neutrophile peptide-1
分类号 Q943.2 [生物学—植物学]
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参考文献20

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二级参考文献55

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