XIAP、XAF1在正常口腔黏膜角化细胞和Tca8113细胞中的表达与定位

Expression and subcellular localization of XIAP and XAF1 in human normal oral keratinocytes and Tca8113 cells

目的明确人正常口腔黏膜角化细胞(hNOK)和舌鳞癌细胞系Tca8113细胞中X染色体连锁凋亡抑制蛋白(XIAP)与XIAP相关因子1(XAF1)的表达与定位情况。方法对hNOK和Tca8113细胞进行体外培养,间接免疫荧光染色结合激光共聚焦显微镜检测细胞中XIAP和XAF1蛋白的表达与亚细胞定位。结果在hNOK中XIAP微弱表达,主要分布于胞质和核周区域,而Tca8113细胞中XIAP呈高强度表达,胞质和胞核中均有分布。在hNOK中XAF1主要分布于胞核内,而Tca8113细胞中XAF1均匀分布于胞质与胞核。结论在口腔黏膜癌变过程中,XIAP可能通过过度表达发挥重要的抗凋亡作用,而XAF1不能有效拮抗XIAP的作用。

Objective To clarify expression and subcellular localization of XIAP and XAF1 protein in human normal oral keratinocytes（hNOK） and Tca8113 cells human tongue carcinoma cell line. Methods The hNOKs and Tca8113 cells were cultured in vitro. Expression and subcellular localization of XIAP and XAF1 protein were examined by combination of indirect immunofluorescence and confocal laser scanning microscopy. Results XIAP expression was weak in the hNOKs and fluorescence staining localized chiefly in the cytoplasm and perinuclear areas. In the Tca8113 cells, high level of XIAP protein could be detected in both the cytoplasm and the nucleus. In the hNOKs, XAF1 distributed mostly in the nucleus. Homogeneous nuclear and cytoplasmic distribution of XAF1 could be visualized in the Tca8113 cells. Conclusions In cancerization of oral mucosa, XIAP protein could play an important antiapoptotic role by overexpression, while XAF1 protein does not appear to antagonize effectively the role of XIAP.