拉米夫定耐药性变异与乙型肝炎病毒基因型间的关系

Researching on the relation between lamivudine-resistant mutation and HBV genotypes

目的探讨乙型肝炎病毒(HBV)基因型与拉米夫定耐药性变异类型间的关系。方法选取95例来自本院肝炎门诊和病房的YMDD变异阳性血清样品,应用限制性内切酶MboⅠ和EarⅠ对PCR产物进行酶切分析及分型,并作遗传进化树图分析验证。结果95例样品中检出C型75例,占79%;B型20例,占21%,进化树图分析证明酶切分型法确切可靠;B型病毒的YVDD变异、YIDD变异及YMDD+YVDD+YIDD联合变异的例数分别为11例、5例和4例,而C型中则分别为35例、27例和13例,对B、C基因型与YMDD变异类型间作卡方检验,得出差异无统计学意义(χ2=0.856,P=0.710)。结论使用MboⅠ和EarⅠ酶切分型操作简单,结果可靠。HBVB型和C型病毒感染对发生不同YMDD变异类型无影响。

Objective To analyze the relation between lamivudine-resistant mutation types and HBV genotypes. Methods 95 cases with YMDD mutation were selected from out-patient clinic and sickroom in our hospital. Restriction endonuclease MboⅠ and EarⅠ were used to identify HBV genotypes of PCR product, farther a phylogenesis tree was applied to check it. Results We could divide 95 cases into two parts, 75 cases（78.95% ） were HBV C genotype and 20 cases（21.05% ） were B genotype, these were verified by phylogenesis tree. With regard to the types of YVDD mutation, YIDD mutation and YMDD ＋ YVDD ＋ YIDD mutation, there were 11 cases, 5 cases, 4 cases in genotype B, respectively, but 35 cases, 27 cases, 13 cases in genotype C. We made the cha-test to check genotypes and types of YMDD mutation, χ^2 =0. 856, P = 0. 710. There were no significant differences. Conclusions Using MboⅠ and EarⅠ can genotype HBV PCR product easily and reliably. It is no statistical significance between HBV B or C genotype and the types of YMDD mutation.