摘要
AIM: To study the effect of albumin administration on lung injury in trauma/hemorrhagic shock (T/HS). METHODS: Sixty experimental animals were randomly divided into three groups: rats undergoing laparotomy without shock (T/SS); rats with T/HS and resuscitation with blood plus twice the volume of shed blood as Ringer’s lactate (RL), and rats with T/HS and resuscitation with blood plus additional 3 mL of 50 g/L human albumin. Expression of polymorphonuclear neutrophil (PMN) CD11b/CD18, intercellular adhesion molecule-1 (ICAM-1) of jugular vein blood and the severity of lung injuries [determined mainly by measuring activity of lung tissue myeloperoxidase (MPO) and lung injury score (LIS)] were measured after a 3-h recovery period. RESULTS: All three groups showed a significant difference in the expressions of CD11b/CD18, ICAM-1, and severity of lung injury. The expressions of CD11b/ CD18 in T/SS group, T/HS + RL group, T/HS + albumin group were 17.76% ± 2.11%, 31.25% ± 3.48%, 20.36% ± 3.21%, respectively (F = 6.25, P < 0.05). The expressions of ICAM-1 (U/mL) in T/SS group, T/ HS + RL group, T/HS + albumin group were 258.76 ± 98.23, 356.23 ± 65.6, 301.01 ± 63.21, respectively (F = 5.86, P < 0.05). The expressions of MPO (U/g) in T/SS group, T/HS + RL group, T/HS + albumin group were 2.53 ± 0.11, 4.63 ± 1.31, 4.26 ± 1.12, respectively (F = 6.26, P < 0.05). Moreover, LIS in T/HS + RL group, T/HS + albumin group was 2.62 ± 0.23, 1.25 ± 0.24, respectively. The expressions of CD11b/CD18, ICAM-1 and MPO in T/HS + RL group were significantly increased compared to T/SS group (P = 0.025, P = 0.036, P = 0.028,respectively). However, administration of 3 mL of 50 g/L albumin significantly down-regulated the expressions of CD11b/CD18, ICAM-1 and lung injury index (MPO and LIS) when compared with the T/HS + RL rats (P = 0.035, P = 0.046, P = 0.038, P = 0.012, respectively). CONCLUSION: The infusion of albumin during resuscitation period can protect lung from injury and decrease the expressions of CD11b/CD18, ICAM-1 in T/ HS rats.
AIM： To study the effect of albumin administration on lung injury in trauma/hemorrhagic shock （T/HS）. METHODS： Sixty experimental animals were randomly divided into three groups： rats undergoing laparotomy without shock （T/SS）; rats with T/HS and resuscitation with blood plus twice the volume of shed blood as Ringer＇s lactate （RL）, and rats with T/HS and resuscitation with blood plus additional 3 mL of 50 g/L human albumin. Expression of polymorphonuclear neutrophil （PMN） CD11b/CD18, intercellular adhesion molecule-1 （ICAM-1） of jugular vein blood and the severity of lung injuries [determined mainly by measuring activity of lung tissue myeloperoxidase （MPO） and lung injury score （LIS）] were measured after a 3-h recovery period. RESULTS： All three groups showed a significant difference in the expressions of CD11b/CD18, ICAM-1, and severity of lung injury. The expressions of CD11b/ CD18 in T/SS group, T/HS ＋RL group, T/HS ＋albumin group were 17.76% ± 2.11%, 31.25% ± 3.48%, 20.36% ± 3.21%, respectively （F = 6.25, P 〈 0.05）. The expressions of ICAM-1 （U/mL） in T/SS group, T/ Hs ＋ RL group, T/HS ＋albumin group were 258.76 ± 98.23, 356.23 ± 65.6, 301.01 ± 63.21, respectively （F = 5.86, P 〈 0.05）. The expressions of MPO （U/g）） in T/SS group, T/HS ＋RL group, T/HS ＋albumin group were 2.53 ± 0.11, 4.63 ± 1.31, 4.26 ± 1.12, respectively （F = 6.26, P 〈 0.05）. Moreover, LIS in T/HS ＋RL group, T/HS ＋albumin group was 2.62 ± 0.23, 1.25 ± 0.24, respectively. The expressions of CD11b/CD18, ICAM-1 and MPO in T/HS ＋RL group were significantly increased compared to T/SS group （P = 0.025, P = 0.036, P = 0.028,respectively）. However, administration of 3 mL of 50 g/L albumin significantly down-regulated the expressions of CD11b/CD18, ICAM-1 and lung injury index （MPO and LIS） when compared with the T/HS ＋RL rats （P = 0.035, P = 0.046, P = 0.038, P = 0.012, respectively）. CONCLUSION： The infusion of albumin during resuscitation period can protect lung from injury and decrease the expressions of CD11b/CD18, ICAM-1 in T/ HS rats.
基金
Supported by the Traditional Chinese Medicine Research Foundation, Zhejiang Province, China 2005C072
