三氧化二砷诱导胶质母细胞瘤细胞凋亡作用的研究

Effect of Arsenic Trioxide on Cells Apoptosis of Glioblastomas

目的探讨三氧化二砷(As2O3)及其联合地塞米松(DEX)诱导胶质母细胞瘤细胞凋亡的作用。方法实验分为空白对照组、As2O3组、DEX组、As2O3+DEX组,经倒置显微镜观察、吖啶橙荧光染色、流式细胞计数、细胞DNA片段断裂率来检测As2O3及其联合DEX诱导胶质母细胞瘤细胞凋亡的作用。结果As2O3以浓度依赖形式诱导BT-325细胞凋亡;DEX具有明显的协同效应。结论As2O3与DEX联合用药可能成为治疗胶质母细胞瘤的一条新途径。

Objective To evaluate the curative effects of arsenic trioxide （As2O3） on cells apoptosis of human glioblastoma cell line, BT-325 in vitro. Methods According to different agents added in the medium of cultured BT-325 cells, cultured glioblastoma cells were divided into As2O3, dexamethasone （DEX）, As2O3＋DEX and blank control groups. Cells apoptosis was assessed by inversion microscope observation, fluorescent staining, flow cytometry and cell DNA fragmentation rate analysis. Results As2O3 induced effectively apoptosis of BT-325 cells in a dose-time dependent manner. Significant synergetic effect of As2O3 and DEX on BT-325 cells apoptosis was observed. Conclusion Administration of As2O3 in combination with DEX may be a new method to treat of glioblastoma.