摘要
目的探讨三氧化二砷(As2O3)及其联合地塞米松(DEX)诱导胶质母细胞瘤细胞凋亡的作用。方法实验分为空白对照组、As2O3组、DEX组、As2O3+DEX组,经倒置显微镜观察、吖啶橙荧光染色、流式细胞计数、细胞DNA片段断裂率来检测As2O3及其联合DEX诱导胶质母细胞瘤细胞凋亡的作用。结果As2O3以浓度依赖形式诱导BT-325细胞凋亡;DEX具有明显的协同效应。结论As2O3与DEX联合用药可能成为治疗胶质母细胞瘤的一条新途径。
Objective To evaluate the curative effects of arsenic trioxide (As2O3) on cells apoptosis of human glioblastoma cell line, BT-325 in vitro. Methods According to different agents added in the medium of cultured BT-325 cells, cultured glioblastoma cells were divided into As2O3, dexamethasone (DEX), As2O3+DEX and blank control groups. Cells apoptosis was assessed by inversion microscope observation, fluorescent staining, flow cytometry and cell DNA fragmentation rate analysis. Results As2O3 induced effectively apoptosis of BT-325 cells in a dose-time dependent manner. Significant synergetic effect of As2O3 and DEX on BT-325 cells apoptosis was observed. Conclusion Administration of As2O3 in combination with DEX may be a new method to treat of glioblastoma.
出处
《中国临床神经外科杂志》
2006年第11期674-677,共4页
Chinese Journal of Clinical Neurosurgery
基金
江西省卫生厅资助课题(课题编号:0301024)
关键词
胶质母细胞瘤
三氧化二砷
凋亡
地塞米松
Glioblastoma
Arsenic trioxide (As2O3)
Apoptosia
Dexamethasone.