摘要
孔雀石绿(MG)和结晶紫(GV)曾作为渔药使用,在鱼体内可分别代谢为无色孔雀石绿(LMG)及无色结晶紫(LGV),具有潜在的致癌性,已被许多国家禁用。本文研究了同时检测孔雀石绿、结晶紫及其代谢物无色孔雀石绿和无色结晶紫的液相色谱方法,建立了检测前处理方法及色谱条件。经过液液萃取和丙基磺酸阳离子树脂纯化,采用C18分离柱后串联二氧化铅柱氧化衍生的方法,同时检测母体药物及其代谢物,回收率>70%,重复性的相对标准偏差<15%,定量限分别为2μg/kg及1μg/kg。经样品实测,在一份鲈鱼样品中检测到无色孔雀石绿残留97μg/kg。
Malachite green (MG) and gentian violet (GV) are potentially carcinogenic triarylemethane dyes and have been prohibited as aquaculture drugs in many countries. Malachite green and gentian violet can be transformed into leucomalachite green (LMG) and leucogentian violet (LGV) respectively in fish muscle tissue. The simultaneous determination of these four substances in fish muscle by high-performance liquid chromatography (HPLC) was studied. After liquld-liquid extraction and PRS (propylsulfonic acid) ion exchange chromatography, the simultaneous determination of the original substances and their metabolites was achieved by means of PbO2 post-column oxidation after C18 column separation. The recoveries for fortified fish tissue were more than 70 %. The relative standard deviation (RSD) values for repeatability were less than 15 %. The limits of quantification were respectively 1 μg/kg and 2 μg/kg. A sample of sea perch was analyzed to contain 97 μg/kg LMG residue.
出处
《中国海洋大学学报(自然科学版)》
CAS
CSCD
北大核心
2006年第6期919-922,共4页
Periodical of Ocean University of China
基金
科技部标准重大专项"主要养殖水产品安全限量及控制标准研究"(2002BA906A39)资助