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液相色谱法测定鱼肌肉中孔雀石绿、结晶紫及其代谢物 被引量:24

Determination of Residues of Malachite Green,Gentian Violet and Their Metabolites in Fish Tissues by High-Performance Liquid Chromatography
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摘要 孔雀石绿(MG)和结晶紫(GV)曾作为渔药使用,在鱼体内可分别代谢为无色孔雀石绿(LMG)及无色结晶紫(LGV),具有潜在的致癌性,已被许多国家禁用。本文研究了同时检测孔雀石绿、结晶紫及其代谢物无色孔雀石绿和无色结晶紫的液相色谱方法,建立了检测前处理方法及色谱条件。经过液液萃取和丙基磺酸阳离子树脂纯化,采用C18分离柱后串联二氧化铅柱氧化衍生的方法,同时检测母体药物及其代谢物,回收率>70%,重复性的相对标准偏差<15%,定量限分别为2μg/kg及1μg/kg。经样品实测,在一份鲈鱼样品中检测到无色孔雀石绿残留97μg/kg。 Malachite green (MG) and gentian violet (GV) are potentially carcinogenic triarylemethane dyes and have been prohibited as aquaculture drugs in many countries. Malachite green and gentian violet can be transformed into leucomalachite green (LMG) and leucogentian violet (LGV) respectively in fish muscle tissue. The simultaneous determination of these four substances in fish muscle by high-performance liquid chromatography (HPLC) was studied. After liquld-liquid extraction and PRS (propylsulfonic acid) ion exchange chromatography, the simultaneous determination of the original substances and their metabolites was achieved by means of PbO2 post-column oxidation after C18 column separation. The recoveries for fortified fish tissue were more than 70 %. The relative standard deviation (RSD) values for repeatability were less than 15 %. The limits of quantification were respectively 1 μg/kg and 2 μg/kg. A sample of sea perch was analyzed to contain 97 μg/kg LMG residue.
出处 《中国海洋大学学报(自然科学版)》 CAS CSCD 北大核心 2006年第6期919-922,共4页 Periodical of Ocean University of China
基金 科技部标准重大专项"主要养殖水产品安全限量及控制标准研究"(2002BA906A39)资助
关键词 液相色谱 孔雀石绿 结晶紫 代谢物 HPLC Malachite green gentian violet metabolites
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参考文献12

  • 1潘方国,杨帆.船体网箱饲养的鲤鱼水霉病发病条件及防治药物筛选[J].水产养殖,1995,16(5):19-21. 被引量:3
  • 2王丙乾,李永发,贾忠贺.大西洋鲑稚鱼(Oncorhynchus solar)小瓜虫病(Lchthyophthirius filiis)的防治试验[J].水产学杂志,2001,14(2):43-46. 被引量:5
  • 3Alborali L,Sangiorgi E,Leali M,et al.The persistence of malachite green in the edible tissue of rainbow trout(Oncorhynchus mykiss)[J].Riv Ital Acquacolt,1997,32:45-60.
  • 4Thompson H C Jr,Rushing L G,Gehring T,et al.Persistence of gentian violet and leucogentian violet in channel catfish (Ictalurus punctatatus) muscle after water-borne exposure[J].J of Chromatography B,1999,723:287-291.
  • 5Rao KVK.Inhibition of DNA synthesis in primary rat hepatocyte cultures by malachite green:a new liver tumor promoter[J].Toxicol Lett,1995,81(2):107-113.
  • 6Littlefield N A.Chronic toxicity and carcinogenicity studies of gentian violet in Fishcher 34 rats[R],NCTR Technical Report for Experiment No.338 National Center for Toxicological Research,Jefferson,AR 72079.
  • 7Roybal J E,Denver C O,Pfenning A P,et al.Determination of malachite green and its metabolite,leucomalachite green,in catfish (Ictalurus punctatus) tissue by liquid chromatography with visible detection[J].J of AOAC,1995,78:453-457.
  • 8郭德华,叶长淋,朱莹洁.液相色谱法测定鳗鱼中的孔雀石绿[J].化学分析计量,2002,11(2):20-21. 被引量:52
  • 9Bergwerff A A,Scherpenisse P.Determination of residues of malachite green in aquatic animals[J].J of Chromatography B,2003,788:351-359.
  • 10Scherpenisse P,Bergwerff A A.Determination of residues of malachite green in finfish by liquid chromatography tandem mass spectrometry[J].Analytica Chimica Acta,2005,529:173-177.

二级参考文献12

  • 1鞠长增,程天印,赵万鹏,鞠波,黄斌,周本.孔雀石绿消毒中华鳖使用浓度和作用时间的研究初报[J].信阳师范学院学报(自然科学版),1994,7(4):417-420. 被引量:2
  • 2Alderman D J.Malachite green:a review[J].J Fish Dis,1985,8:289-298.
  • 3Steven M Plakas,Kathleen R El Said,Guy R Stehly,et al.Optimization of a liquid chromatographic method for determination of malachite green and its metabolites in fish tissues[J].J of AOAC,1995,78:1388-1394.
  • 4Jose E Roybal,Allen P Pfenning,Robert K Munns,et al.Determination of malachite green and its metabolite,leucomalachite green,in catfish (Ictalurus punctatus) tissue by liquid chromatography with visible detection[J].J of AOAC,1995,78:453-457.
  • 5Daniel R Doerge,Hebron C Chang,Raoo L Divi,et al.Mechanism for inhibition of thyroid peroxidase by leucomalachite green[J].Chem Res Toxicol,1998,11:1098-1104.
  • 6Larry G Rushing,Harold C Thompson Jr.Simultaneous determination of malachite green,gentian violet and their leucometabolites in catfish or trout tissue by high-performance liquid chromatography with visible detection[J].J of Chromatography B,1997,688:325-330.
  • 7Allen J L,Meinertz J R.Post-column reaction for simultaneous analysis of chromatic and leuco forms of malachite green and crystal violet by high-performance liquid chromatography with photometric detection[J].J Chromatography B,1991,536:217-222.
  • 8王声瑜.怎样有效选用含氯消毒剂和孔雀石绿[J].北京水产,1999(3):16-16. 被引量:22
  • 9袁定清.欧洲鳗的小瓜虫病及其防治[J].水产科技情报,2000,27(1):46-48. 被引量:5
  • 10卢迈新,黄樟翰,肖学铮,吴锐全,谢骏.美洲鳗对几种药物的敏感性研究[J].淡水渔业,2000,30(5):28-29. 被引量:59

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