摘要
从羊痘病毒基因组中寻找并扩增羊白细胞介素-18结合蛋白(IL-18BP)基因。将该基因克隆到表达载体PGEX-5X-3,在大肠杆菌BL21中实现了高效表达。目的蛋白以包涵体形式存在。表达产物经过变性、复性和层析纯化后,具有体外拮抗羊IL-18刺激外周血单核细胞产生IFN-γ的活性。
Some virus genome of vaccinia subgenus in poxvirus encoded IL-18BP gene, which can block IL-18 activity but no sequence similarity to membrane IL-18 receptors. Virus genome in sheep pox subgenus can also be induced having this gene. A pair of primers spanned the ORF of goat IL-18BP gene in sheep pox virus genome was designed. We cloned this gene and expressed it in E. Coli with pGEX-5X-3 plasmid. After being purified, the recombinant fusion protein can inhibit goat IL-18 and induce goat PBMC IFN-γ production in goat PBMC.
出处
《山东科学》
CAS
2006年第6期53-55,共3页
Shandong Science
基金
聊城大学博士基金(31805)
