试剂红细胞的研究与应用

Study and Application of the Reagent Red Blood Cells

目的：研制试剂红细胞用于血型鉴定反定型，保证临床输血安全。方法：制备效期长的红细胞保养液，再用该保养液制备4％的试剂红细胞，2℃-8℃冰箱保存，每周对其进行红细胞抗原性和免疫特异性测定及反定型试验．观察细胞凝集状况。结果：本法制备的试剂红细胞保存期为68d，具有较好的抗原特异性和细胞稳定性，68d以后凝集强度减弱。结论：该试剂红细胞凝集效能良好，红细胞抗原稳定性、免疫特异性好，具有成本低，制作易，操作简便等优点。

Objective To study the reagent red blood cells which is used in the reverse typing of ABO insure the safety of clinical transfusion. Methods We make the nutrient liquor for keeping the red cell alive, and by using this nutrient liquor to produce the 4% the reagent rde blood cells that is kept under the 2℃～8℃ in the refrigerator. Every week we test the antigen, immunological specificity and dosome reverse typing experiments of the rde cells with the 4% the reagent red blood cells in order to observe the condensation of cells. Results The reagent red blood cells can exist sixty-eight days, by this method we have the better antigen specificity and stability of cells. After sixty-eight days the intensity of condensation will be brought down. The condensation of this reagent red blood cells is fine, the immunological specificity and the stable antigen of the red cells is good. At the same time this method have many advantages, such as the easy method and operation, the cheaper expense and so on.