摘要
利用去壁低渗染色体计数法及流式细胞术法,首次系统地对中国野生葡萄18个种64个株系的染色体倍性进行了鉴定。结果表明,供试的中国野生葡萄全部为二倍体(2n=38)。同时也证明流式细胞仪不仅可以进行染色体倍性的鉴定,且制样简单、测定快速、结果可靠。
Ploidy of 64 clones of 18 wild Chinese wild Vitis species were studied by means of the wall degradation hypotonic method and Flow Cytometry in order to get basic information about the inheritance, variance, evolution and variety character of Chinese wild Vitis. The results showed that all of the 64 observed clones were diploids. The results also showed that Flow Cytometry could be used for identifying ploid and was the ideal method because of the simple preparation of sample, rapid and accurate measurement.
出处
《西北农业学报》
CAS
CSCD
北大核心
2006年第6期148-152,共5页
Acta Agriculturae Boreali-occidentalis Sinica
基金
国家自然科学基金项目(No.30370993No.30571280)资助