小鼠TIL、LAK细胞穿孔蛋白mRNA表达与其抗瘤作用的关系

THE STUDY ON THE RELATION BETWEEN INTRACELLULAR PFP mRNA EXPRESSION AND ANTITUMOR ACTIVITY IN VITRO AND IN VIVO OF TILs AND LAK CELLS OF MOUSE

用原位杂交法检测小鼠不同培养时期的ＴＩＬ和ＬＡＫ细胞中穿孔蛋白（ＰＦＰ）ｍＲＮＡ的表达，并用显微图象分析系统处理检测ＰＦＰｍＲＮＡ表达水平，结果表明：ＴＩＬ细胞的ＰＦＰｍＲＮＡ表达水平在第１４、２１天时达高峰，２８天时开始下降；而ＬＡＫ细胞则在第７天时即达到较高水平，２１天时开始下降，但ＴＩＬ细胞的表达水平显著高于ＬＡＫ。将同时期的ＴＩＬ及ＬＡＫ细胞进行体内外抗瘤活性测定，发现两者的ＰＦＰｍＲＮＡ表达水平，均与其体外杀伤活性有明显的相关性，而体内抗瘤活性与体外杀伤活性间则无显著相关性。研究结果提示，ＰＦＰ在小鼠ＴＩＬ，ＬＡＫ细胞介导的溶细胞过程中起着重要作用，而ＴＩＬ和ＬＡＫ细胞的体内抗瘤活性还受体内多种因素的影响。

The level of PFP mRNA expression in TILs and LAK cells of mouse during various culture time was investigated with hybridization in situ and computer-microphotography analysis system. The results showed that the quantity of PFP mRNA expression in TILs increased to the highest level on cultured 14th to 21th day, and then declined to lower level on 28th day, nevertheless, that in LAK cells got to higher level on 7th day and then dropped to lower level on 21st day, In addition, the level of PFP mRNA expression in TILs was remarkably higher than that in LAK cells, Meanwhile, we detected the antitumor activity of these TILs and LAK cells in vivo and in vitro, It was found that there was significant correlation between the level of PFPmRNA expression and the cytotoxicity in vitro in TILs as well as LAK cells, but there was no notable correlation between the cytotoxicity in vitro and antitumor activity in vivo of both TILs and LAK cells, According to above results, it may be inferred that PFP plays an important role in cytotoxicity of TILs and LAKcells, and the antitumor activity in vivo of TILs and LAK cells is affected by various factors besides PFP.