侵袭性牙周炎的表观遗传学研究

Study of Epigenetics in Aggressive Periodontitis Gingival

目的探讨侵袭性牙周炎的表观遗传学特征及相关基因表达特征。方法收集重度侵袭性牙周炎女性患者36例及35名无牙周炎女性对照者的牙龈组织,采用标准酚-氯仿抽提法和蛋白酶K消化法提取DNA。用甲基化敏感的限制性内切酶PCR法测定TIMP1基因的甲基化修饰情况,分析对照组与患者组基因表达的差异。结果重度女性侵袭性牙周炎患组中两条X染色体上TIMP1基因都处于去甲基化状态的比例高于对照组,差异有统计学意义。结论TIMP1基因表观遗传学特征改变可能与侵袭性牙周炎的易感性有关。

Objective To investigate the association between TIMP1 gene polymorphic expression and susceptibility to aggressive periodontitis. Methods Gingiva from 36 patients with severe aggressive periodontitis and 35 matched control individuals were collected. DNA was extracted from gingival by phenol -chloroform standard, and proteinase K was used to digest proteins. Methylation - sensitive restriction PCR was used to determine whether TIMP1 gene is methylated or not. Different expression of the TIMP1 gene between patients and controlled groups were analyzed. Resldts Property of unmethylated TIPM1 gene linked to two X chromosome in female severe aggressive periodontitis patients is higher than that of the controlled group. The difference was statistically significant. Conclusion These data indicate that polymorphic expression of TIMP1 gene may be associated with the susceptibility to aggressive periodontitis,