摘要
目的:研究罗通定在大鼠胆汁中的主要代谢产物。方法:灌胃给药后,收集大鼠胆汁,液液萃取,采用HPLC/MS/MS法分析鉴定罗通定的代谢产物。色谱柱Lichrospher-C18(5μm,4.6mm×250mm),流动相:甲醇-水-三乙胺(37630.063,V/V/V),冰醋酸调pH至6.3,检测波长281nm。结果:在大鼠胆汁中发现2个罗通定代谢产物,初步推测其结构为罗通定单羟基化后再与葡萄糖醛酸结合的产物。结论:罗通定在大鼠胆汁中主要以羟基化后的葡萄糖醛酸结合物形式排泄。
AIM： To investigate the main metabolites of mttmdine in rat bile. METHOD： Bile of rats after oral administration of rotundine （200 mg·kg^-1） was analysed by HPLC/MS/MS. Chromatographic analysis was performed on a Lichrospher-Cl8 column （5μm, 4.6mm×250 mm）; mobile phase： methanol-water-triethylamine （37 ： 63 ： 0.063, V/V/V）, adjusting pH to 6.3 with acetic acid. UV detector was set at 281 nm and positive ion mode was operated. RESULT： Two metabolites were found in rat bile, whose structure may be glucuronide conjugates of rotundine after demethylation. CONCLUSION： Conjugate with glucuronic acid after demethylation may be the main excretion pathway of rotundine in rat bile.
出处
《中国天然药物》
SCIE
CAS
CSCD
2006年第6期448-451,共4页
