血清载脂蛋白参考值

Referencevaluesofserumapolipoproteins

目的调查健康中、老年人载脂蛋白（ａｐｏ）ＡＩ、ＡⅡ、Ｂ、ＣＩ、ＣⅡ、ＣⅢ、Ｅ及脂蛋白（ａ）［Ｌｐ（ａ）］的参考值，分析各项血脂与ａｐｏ之间的相关。方法严格选择生活水平一般的北京健康居民，男２２５人，女５８人，年龄３８～８３岁（大都在４５～７０岁间）。血脂测定用标准化方法，ａｐｏＡＩ、Ｂ参考血清的定值符合ＷＨＯ国际标准。除Ｌｐ（ａ）与ａｐｏＣＩ测定用ＥＬＩＳＡ法外，其他各项ａｐｏ分析均用免疫透射比浊法。结果统计得各项ａｐｏ的均数与百分位数分布并与国内外资料比较。相关分析显示Ｌｐ（ａ）与ａｐｏＣＩ是独立的变量，与年龄、体重及各项血脂均无明显相关。其他各项血脂与ａｐｏ指标可以分为３组，分别代表高密度、低密度与极低密度脂蛋白水平。分析了体重指数、甘油三酯与高密度脂蛋白的相互关系。结论ａｐｏＡＩ、Ｂ测定中采用了ＷＨＯ国际参考血清后结果比以往的报告有明显提高。其他ａｐｏ测定尚未开始标准化研究，但作者所列参考值大致与用同类方法所报告的数据近似。１４项血脂之间的相关分析。

sObjectivesToinvestigatetheeferencevaluesofapoAI,AⅡ,CIⅠⅡ,CⅢ,Eandlipoprotein(a)ofmiddleagedandoldsubjects,andtostudythecorrelationsbetweenvari-ouslipidparameters.MethodsAfterthoroughphysicalandlaboratoryexamination,225maleand58femalehealthyBeijingresidents(aged38-83,mostly45-75yearsold)withmoderatelivingstandardwereselected.Serumtotalcholesterol(TC),lipoproteincholesterol(HDL-CandLDL-C)andtriglyceride(TG)wereanalyzedwithwelstandardizedmethods.AssignedvaluesofapoAI,BofserumcalibratorswereprovedtobetrceabletothetargetvaluesofWHOinternationalreferencematerials.Mostaposweredeterminedbyimmunoturbidimetricmethods,butLp(a)andapoCIweredeterminedbyELISAmethod.ResultsAverageapoBlevelswere1.01g/Linmenand1/07g/Linwomen,thecorespondingTCandLDL-Cvalueswere4.87and2.95mmol/Linmen,and5.16and3.13mmol/Linwomenrespectively.MeanapoAIlev-elswere1.42g/Linmenand1.45g/Linwomen,andthecorrespondingHDL-Cwas1.41and1.54mmol/L.TheproportionofHDL2-CandHDL3-Cwasapproximately4:6.ThemeanandmediumvaluesofLp(a)inmaleswere144and107mg/L,infamaleswere154and117mg/L.Themeanandthepercentiledistributionoftheaposstudiedwerelistedindetail.CorrelationanalysisrevealedthatLP(a)andapoCIwereindependentvariables.Otherparame-tersweredividedinto3groups;theymainlyrepresentedthelevelsofHDL,LDL,andVLDL.BodymassindexwasnegativelycorrelatedwithHDL-C,andpositivelycorrelatedwithTG.TGshowedclosenegativecorrelationwithHDL-C,apoAIandapoAⅡ.ConclusionsAl-thoughstandardizationstudyonotheraposhasnotstartedyet,theobservedreferencevaluesofLP(a),aopAⅡ,CsandEweresimilarofthosereportedwiththesamekindofanalyticalap-proach.Simultaneusdeterminationof14lipidparmetersandanalysisoftheircorrelationsmaycontributetothestudyofrelatedmetabolicproblemsandproperselectionoflaboratorytestsforclinicaluse.