摘要
目的为临床合理选择抗厌氧菌药物提供参考依据。方法应用ＶＩＴＥＫ－ＡＭＳ的ＡＮＩ厌氧鉴定卡进行厌氧菌鉴定；用琼脂稀释法测定甲硝唑、苯酰甲硝唑羧苄青霉素及氯霉素对１１５株厌氧菌的最低抑菌浓度（ＭＩＣ）。结果甲硝唑及其衍生物苯酰甲硝唑对拟杆菌的ＭＩＣ９０均为２～４μｇ／ｍｌ，对梭杆菌ＭＩＣ９０分别为１．０μｇ／ｍｌ和０．５μｇ／ｍｌ，；甲硝唑及苯酰甲硝唑对革兰阳性厌氧菌的抗菌活性低于氯霉素和羧苄青霉素。结论４种药物对厌氧菌的ＭＩＣ值有不同程度的上升趋势，但甲硝唑及其衍生物仍可作为抗厌氧菌感染的首选药物，且具有副作用小。
ObjectiveTodetecttheminimalinhibitoryconcentrationsof4antianaerobedrugsa-gainst115anaerobeisolatesinrecenttwoyears,astoinvestigatingthechangesofresistanceofanaerobe,providingphysicianswiththebestselectionofmedicine,andpreventingtheocurrenceofnosocomialinfections.MethodsVITEK-IMSANIcardandagardilutionmethodwereusedfori-dentifyingtheanaerobeanditsminimalinhibitoryconcentrations.ResultsTheresultsshowedthatmetronidazolumhasbeteractivitiesagainstmostanaerobes.TheMIC90forbacteroideswas2μg/ml.Buttheactivityofmetronidazolumwaslowerthanthatofcholoromphnicolandcarbenicilinumagainstgram-positiveanaerobes,especialyagainstactinomycesspecies.ConcusionsTheinvestiga-tionshowsthattheIMCofanaeroberespondingtothe4anti-anaerobedrugswasincreasedatdif-ferentlevels,however,metronidazolumwasstilproposedasthefirstcandidatedrugofanaerobein-fection.
关键词
细菌
厌氧
甲硝唑
微生物
敏感性试验
Bacteria,anaerobicMetronidazoleMicrobidsensitivitytests
