摘要
目的建立在流式细胞仪上利用细胞分化抗原ＣＤ４５（ＣＤ４５）和侧向角光散射（ＳＳＣ）坐标图辅助设门的方法，确保在白血病细胞免疫分型时设门的准确无误。方法根据ＣＤ４５在所有白细胞上都有表达，但在不同系成熟细胞和同系不同发育阶段细胞表面表达密度不同的特点，在不同荧光抗体检测组合中配以ＣＤ４５荧光单克隆抗体，分析前先按常规在前向角光散射（ＦＳＣ）与ＳＳＣ坐标图中设门，再利用ＣＤ４５－ＳＳＣ图辅助设门选择低ＣＤ４５表达细胞群，并以此门返回检查ＦＳＣ－ＳＳＣ图，确认这群细胞恰好落在门中后再进行免疫表型分析。结果通过对１０例正常骨髓；髓系白血病Ｍ１～Ｍ７，慢性粒细胞性白血病，慢性单核细胞性白血病；淋巴系白血病急性淋巴细胞性白血病与慢性淋巴性白血病及多发性骨髓瘤等３００例实例分析，证实该方法易于掌握，重复性好，可正确捕获病理细胞群。结论ＣＤ４５辅助设门提高了白血病免疫学分型的准确性，特别对白血病残余病变。
ObjectiveToestablishanewauxiliarygatingmethodonCD45-sideanglelightscatter(SSC)dimensionalprojection,whichisusefulforroutineaccurategatingleukemiccels.MethodsCD45antigenexpressedonalleukocytes,buttheamountofCD45wasdiferentinseparatelineagesofcelsandalsochangedduringthematurationofcelswithinasinglelineage.Basedonthisobser-vation,weusedCD4monoclonalantibodyindiferentpanelsofimmunofluorescencestainingandconfirmedtheroutinegateonforwardanglelightscatter(FSC)versusSSChistogrambytheauxil-iarygateonCD45versusSSCdisplayforgatingtheflowcytometricdata.ResultsTencasesofnor-malbornmarrowspecimensand300casesofmyeloidleukemiaM1～M7,CMLandCMML,lym-phoidleukemiaALLandCLL,aswelasmyolomawereanalyzedbythisprocedure.Thepositionsofthepopulationwerehighlyreproducible.Thepaternspermitedidentificationofnormalcelsandalowedatentiontobefocusedontheappearanceofpopulationinabnormalposition.ConclusionsRCD45auxiliarygatingisusefulforleukemiccelimmunophenotypeanalysisandmonitoringtheresidualdisease,remissionandrelapse.
