摘要
采用PCR方法对云南汉族人群的ApoB位点进行了Amp—FLP分析,检出12个等位基因,26种基因型.扩增片段长度分布于600~1100bp之间,等位基因频率范围为0.0039~0.4648,杂合度为66.41%,DP值为0.8610.研究结果表明,ApoB位点在云南汉族人群中具有高度多态性分布,在法医学及其他学科领域里均具有较高的应用价值.此外,本研究建立的PCR实验方法将有利于在基层部门推广应用.
PCR method was applied to the analysis on amplified fragment length poly-morphism(Amp~FLP)of Apo B locus among the Han people in Yunnan Province.Twelvealleses and twenty-six genotypes were identified in 128 unrelated individuals.The length ofamplified fragments was distributed between 600 and 1100 bp;the allese frequency covered0.003 9 0.464 8;the heterozygosity was 66.41%;and the discrimination power(DP)was 0.861 0.As the result indicated, Apo B locus took on a highly polymorphism distribu-tion among the Han nationality in Yunnan and possessed a widely applicable value in forensicmedicine as well as in other scientific fields,In addition,PCR method established in thestudy will be beneficial to the broad application at the primary level units.
出处
《昆明医学院学报》
1996年第2期9-11,17,共4页
Journal of Kunming Medical College
基金
云南省应用基础研究基金
关键词
APOB
位点
扩增片段长度
多态性
基因
法医学
Apoliporprotein B(Apo B ),Amplified fragment length polymorphism(Amp-FLP),Variable number tanden repeat(VNTR),Allese frequency