淫羊霍苷诱导肝癌SMMC-7721细胞调亡的实验研究

Experimental Study on Icariin Induced Apoptosis of Cultured Liver Cancer Cell SMMC-7721

目的:研究淫羊霍苷诱导肝癌SMMC-7721细胞调亡。方法:电镜观察淫羊霍苷作用后肝癌SMMC-7721细胞的形态变化;MTT法检测淫羊霍苷对细胞增殖的影响,同时应用流式细胞仪检测细胞凋亡率。结果:MTT法检测结果显示,淫羊霍苷对人肝癌SMMC-7721细胞抑制率的IC50为3.93μg/mL;形态学可观察到细胞表现为核染色质致密浓缩,核碎裂、边聚等,或可见凋亡小体,正常对照则无此表现;流式细胞仪检测结果显示,与正常对照组比较,淫羊霍苷随着浓度的增高,细胞调亡率逐渐增高(P<0.01)。结论:淫羊霍苷可诱导肝癌SMMC-7721细胞调亡。

Objective：To investigate if Icariin induce apoptosis in SMMC-7721 cells. Methods. Inhibitions of proliferation was measured using MTT assay. The amount of apoptotic cells was measured by flow cytometry. And the cellular changes were observed with electron microscope. Results.Icariin elicited typical apoptosis morphological changes. The Icariin inhibited SMMC-7721 cells proliferation in concentration-dependent nlanner,the IC50 was 3.93 μg/mL. And the apoptotic peak was detected by flow cytometry. Conclusion：Icariin can induce apoptosis of the cultured liver cancer cell SMMC-7721.