骨髓增生异常综合征患者骨髓VEGF和bFGF水平的变化及其意义

Clinical Significance of the Vascular Endothelial Growth Factor and Basic Fibroblast Growth Factor Expression in the Patients with Myelodysplastic Syndromes

目的:探讨骨髓增生异常综合征(MDS)患者骨髓浆液中血管内皮细胞生长因子(VEGF)和碱性成纤维细胞生长因子(bFGF)水平变化及其临床意义。方法:采用酶联免疫吸附法(ELISA法)检测38例MDS、10例急性非淋巴细胞白血病(ANLL)患者和15例正常人骨髓浆液的VEGF和bFGF水平,用酶标仪测定VEGF及bFGF光密度平均值。结果:MDS组和ANLL组骨髓浆液VEGF和bFGF水平均显著高于正常对照组(P<0.05,P<0.01),而MDS组VEGF水平显著低于ANLL组(P<0.05),但MDS组bFGF水平与ANLL组之间差异无统计学意义(P>0.05)。骨髓浆液VEGF和bFGF表达与MDS患者性别、年龄、外周血中白细胞、血小板及血红蛋白含量及骨髓涂片中原始细胞百分比(高危组)均无相关(r分别为0.160、0.154、0.193、0.185、0.171、0.286;r分别为0.141、0.175、0.152、0.163、0.158、0.239,P均>0.05)。结论:MDS患者骨髓微环境中bFGF和VEGF表达水平均升高,可能在MDS骨髓血管新生及其病情发展过程中发挥了重要作用。

Objective：To investigate the vascular endothelial growth factor （VEGF） and basic fibroblast growth factor（bFGF） levels in bone marrow and their clinical significance in patients with myelodysplastic syndromes （MDS）. Methods. The VEGF and bFGF concentrations were measured using an enzyme-linked immunosorbent assay in bone marrow samples of 38 cases of MDS and 30 cases of the control which included 15 cases of acute non-lymphocytic leukemia（ANLL）, 15 cases of normal volunteers. Results：The VEGF and bFGF levels in bone marrow fluid of patients with MDS and ANLL were higher than that in normal controls（P〈0.05） ,and the VEGF level in MDS group was lower than that in ANLL group（P〈0. 05）. But there was no difference between the bFGF level in MDS group and that in AML group （P2〉0.05）. There was no correlation between VEGF and bFGF levels and the percentage of blasts in the bone marrow of patients with high-risk MDS. Conclusion：VEGF and bFGF may play a role in the angiogenesis and pathophysiology of MDS and anti-angiogenesis could constitute a novel strategy for the treatment of MDS.