实验性人大肠癌裸鼠肝转移模型的建立

Establishment of nude model for human colorectal cancer liver metas-tasis

用Ｌｏｖｏ和ＨＴ２９人大肠癌细胞株接种于裸小鼠脾被膜下和盲肠壁内，在ＳＰＦ条件下饲养动物，建立人大肠癌裸小鼠肝转移模型，并比较两种接种途径的优缺点。结果表明，经脾接种癌细胞建立的人大肠场肝转移模型，方法简单，成功率高，并能一定程度地反映癌细胞的恶性程度和转移潜能。因此，经脾接种场细胞造模是建立实验性人大肠癌肝转移模型较理想的途径。

In order to estaHish a simple and more enective nude mouse model for human colorectal cancerliver metastasis, models of intra-spleen and cecal wall injection of two human colorectal cancer cell lines(HT29 and Lovo),were employed and compared under specific pathogen free(SPF)condition. The resultsshowed that intra-spleen injection model was a successful and simple one for liver metastasis of human col-orectal cancer. This model can reflect tumor malignancy and metastasis potential in some degree. The datasuggest that intra-spleen injection of tumor cells is a proper way for the establishment of experimentalmodel of human colorectal cancer liver metastasis.