用斑点免疫结合法测定登革病毒单克隆抗体亲和力

Affinity measurement of monoclonal antibody to NSl of Dengue VirusⅡby dot immunobinding assay

建立斑点免疫结合法（ＤＩＡ）测定抗体相对亲和力。用此法出定了登革病毒Ⅱ型非结构蛋白ＮＳ１四株单克隆抗体（ＭｃＡｂｓ）的相对亲和力，４株ＭｃＡｂｓ对ＮＳ１的亲和力各不相同。按５０％最大结合浓度分析，抗体株６─１／Ｃ为０．０５μｇ／ｍｌ．１─７／Ｆ为０．６７μｇ／ｍｌ．５Ｄ为２５．００μｇ／ｍｌ，３─１１／Ｃ为５７．００μｇ／ｍｌ。以上结果为应用这些单克隆抗体提供了依据。同时，说明用ＤＩＡ法测定ＭｃＡｂ相对亲和力的可行性。

A new method was developed using Dot Immunobinding Assay(DIA)to measure the affinity ofantibodies,Affinity of four monoclonal antibodies(McAbs)o NSl of Dengue virus Ⅱ was measured withthis new method.Consequently,the affinity of fotir McAbs to NSl is different from each other.The proteinconcentration of 50% maximum binding of the McAbs of 6-1/C,1-7/F,5/D and 3-11/C ws0.05μg/ml,0.67μg/ml,25.00μg/ml and 57.00μg/ml respectively. This study is helpful to make rationaluse of these McAbs. At the same time,it is concluded that the relative affinitv of antibodies can be mea-sured by DIA.