稳定表达MnSOD细胞系的建立及对电离辐射损伤的作用

Establishment of cell lines stably expressing human manganese super-oxide dismutase and its effect on ionizing radiation injury

通过基因转染技术将人ＭｎＳＯＤｃＤＮＡ载体导入中国仓鼠卵巢（ＣＨＯ）细胞，建立了稳定表达有义和反义ＭｎＳＯＤ的细胞系，并用此细胞模型初步探讨了ＭｎＳＯＤ在电离辐射损伤中的作用，从正反两个方面说明了ＭｎＳＯＤ对电离辐射敏感性的影响。

Manganese superoxide dismutase(MnSOD)is the most important antioxidant enzyme mainly lo-cated in mitochordria, MnSOD can protect cells from reactive oxygen species(ROS)injury by scanvengingOigenerated in the respiratory chain. Cell lines stably expressing human MnSOD cell lines were establishedby transfecting Chinese hamster ovary(CHO) ceIls with sense and antisense human MnSOD cDNA vec-tors. It is known that ionizing radiation could induce ROS,thus we investigated the role of MnSOD in ioniz-ing radiation injury and demonstrated the effect MnSOD on radiosensitivity. It is shown that overexpressionof MnSOD could protect and promotes the survival of CHO cells from ionizing radiation.