利用同源重组技术调节啤酒中SO_2含量研究

The Study on Regulation of Sulphur Dioxide in Beer by Homologous Recombination Techniques

二氧化硫在啤酒中具有抗氧化的重要功能,亚硫酸盐还原酶(MET10编码)在啤酒酵母硫代谢过程中起重要作用。利用同源重组技术,采用醋酸锂转化法,将一段目的基因转入到酵母体内,从而获得一株亚硫酸盐还原酶基因突变的工业酿酒酵母。突变株通过驯养,对比发酵栓试验,结果表明在发酵结束时,突变菌株的SO2产量是出发菌株的1.5倍。

Sulphite is an important to stabilize the beer flavor because of its antioxidant activity. Sulfite reductase （encoded by MET10） plays important role in the process of sulphur metabolism. With the application of homologous recombination techniques,a resistant gone was transformed to Saccharomyces cerevisiae genome so as to inactivate MET10 by LiAc method and a new mutant strain was ahtainedo After domestication of mutant strain, a small scale fermentation was carried out. At the end of fermentation, the sulphite produced by the mutant strain was 1.5 fold as much as that of the original strain.