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甲基硝基亚硝基胍诱导CHL细胞中γH2AX焦点的形成 被引量：1

N-methyl-N'-nitro-N-nitrosoguanidine Induces the Phosphorylation of Histone H2AX in CHL Cells
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摘要 背景与目的:研究甲基硝基亚硝基胍(MNNG)是否可诱导中国仓鼠肺成纤维细胞(CHL)中γH2AX焦点的形成,以及是否有细胞系依赖性。材料与方法:应用MTT试验检测不同浓度MNNG对鼠CHL细胞的生存率的影响;应用免疫荧光实验检测细胞中γH2AX焦点的形成。结果:MTT检测表明MNNG对CHL细胞的细胞毒性有明显的时间和剂量效应;免疫荧光实验发现1mg/LMNNG处理2h、8h、24h的CHL细胞中含有γH2AX焦点的细胞比例及焦点数均较对照组显著增加(P<0.05),含有超过30个γH2AX焦点的细胞比例分别达到56%,92%和91%。结论:MNNG可以诱导CHL细胞中γH2AX焦点的形成,并且有明显的时间效应。 BACKGROUND＆AIM： To study whether N-methyl-N＇ -nitro-N-nitrosoguanidine（MNNG） could induce the formation of γH2AX foci in CHL cells. MATERIALS AND METHODS： The cytotoxic effects of different concentrations of MNNG were evaluated by MTT test. The phosphorylation of H2AX was assessed by using immunofluorescent microscopy. PESULTS： MNNG exhibited a dose- and time-dependent cytotoxic effect on CHL cells. MNNG treatment（1 mg/L） significantly increased the ratio of γH2AX-positive cells and the number of foci/cell at 2, 8, and 24 h. The ratios of cells with over 30 foci/cell were about 56%, 92% and 91%, respectively. CONCLUSION： MNNG could induce the phosphorylation of H2AX in CHL cells in a time-dependent manner.
作者 石为 余艳柯 朱心强 杨军
机构地区 浙江大学医学院病理与病理生理学教研室 浙江大学公共卫生学院毒理学研究所
出处 《癌变．畸变．突变》 CAS CSCD 2006年第6期417-419,共3页 Carcinogenesis,Teratogenesis & Mutagenesis
基金 国家高技术研究发展规划(863)项目(No.2004AA649120) 霍英东基金会高校青年教师基金(101036) 教育部新世纪优秀人才支持计划(NCET_05_0520)
关键词 MNNG TH2AX DNA损伤 遗传毒物 MNNG γH2AX DNA damage genotoxin
分类号 R994.6 [医药卫生—毒理学]
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参考文献10

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同被引文献11

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癌变．畸变．突变
2006年 第6期

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