摘要
背景与目的:研究甲基硝基亚硝基胍(MNNG)是否可诱导中国仓鼠肺成纤维细胞(CHL)中γH2AX焦点的形成,以及是否有细胞系依赖性。材料与方法:应用MTT试验检测不同浓度MNNG对鼠CHL细胞的生存率的影响;应用免疫荧光实验检测细胞中γH2AX焦点的形成。结果:MTT检测表明MNNG对CHL细胞的细胞毒性有明显的时间和剂量效应;免疫荧光实验发现1mg/LMNNG处理2h、8h、24h的CHL细胞中含有γH2AX焦点的细胞比例及焦点数均较对照组显著增加(P<0.05),含有超过30个γH2AX焦点的细胞比例分别达到56%,92%和91%。结论:MNNG可以诱导CHL细胞中γH2AX焦点的形成,并且有明显的时间效应。
BACKGROUND&AIM: To study whether N-methyl-N' -nitro-N-nitrosoguanidine(MNNG) could induce the formation of γH2AX foci in CHL cells. MATERIALS AND METHODS: The cytotoxic effects of different concentrations of MNNG were evaluated by MTT test. The phosphorylation of H2AX was assessed by using immunofluorescent microscopy. PESULTS: MNNG exhibited a dose- and time-dependent cytotoxic effect on CHL cells. MNNG treatment(1 mg/L) significantly increased the ratio of γH2AX-positive cells and the number of foci/cell at 2, 8, and 24 h. The ratios of cells with over 30 foci/cell were about 56%, 92% and 91%, respectively. CONCLUSION: MNNG could induce the phosphorylation of H2AX in CHL cells in a time-dependent manner.
出处
《癌变.畸变.突变》
CAS
CSCD
2006年第6期417-419,共3页
Carcinogenesis,Teratogenesis & Mutagenesis
基金
国家高技术研究发展规划(863)项目(No.2004AA649120)
霍英东基金会高校青年教师基金(101036)
教育部新世纪优秀人才支持计划(NCET_05_0520)