大豆异黄酮对去卵巢高脂模型大鼠PPARδ基因表达的影响

Effect of Soy Isoflavones on Expression of PPARδ in A Menopause Model Rat

背景与目的:研究高含量大豆异黄酮(Soy isoflavones,SI)对去卵巢动脉粥样硬化大鼠PPARδ基因表达的影响。材料与方法:将60只SD大鼠去卵巢,10只喂基础饲料,另50只喂4周高脂饲料后,随机分为高脂模型组,SI低、中、高三个剂量组和雌激素已烯雌酚对照组,共5组,每组10只。继续喂高脂饲料22周,各SI剂量组加喂总异黄酮含量为90.04%,金雀异黄素(genistein,Gen)含量为39.37%的SI,按30mg/kg、90mg/kg、270mg/kg三个剂量饲喂,观察实验大鼠主动脉及肝脏的形态学变化,并检测大鼠肝脏、小肠、主动脉中PPARδ的表达情况。结果:高脂模型组主动脉和肝脏脂质堆积,细胞变形。而SI高剂量组主动脉和肝脏脂质堆积较少,细胞较正常。SI使大鼠肝脏、小肠、血管中PPARδ的表达增高。结论:SI可对抗高脂饲料对去卵巢大鼠造成的脂代谢紊乱,对主动脉壁及肝细胞具有保护作用,且呈剂量-效应关系。SI促进高脂饲料喂养的去卵巢大鼠血管的PPARδ表达。

BACKGROUND ＆ AIM： To study the effect of soy isoflavones on PPARδ expression in a menopause rat model where the ovaries were removed. MATERIALS AND METHODS： Sixty female SD rats without ovary and weighing 200±20g before the experiment were studied, 10 were fed normal diet as normal control（without soybean）,50 were fed high lipid diet containing high cholesterol, high fat and high cholic acid salt for 4 weeks, then divided into 5 group randomly： 3 of these groups were fed high lipid diet with different levels of isoflavone, 30 mg/kg, 90 mg/kg and 270 mg/kg, one group was fed high lipid diet with 0.25 mg/kg estrogen as estrogen control（EC） because of the estrogen effect of isoflavone, and one group was fed high lipid diet all the time only to serve as a atherolsclerosis（AS） model. After 22 week, rats were killed and the pathological changes on the aortic wall and liver were examined and the expression of PPARδ in aortic wall, small intestine and liver were assessed. RESULTS： The aortas and liver of AS model rats were stacked with fat, and the cells were distorted. The groups which were fed high SI or estrogen had less distortion. The expression of PPARδ varied in different groups. CONCLUSION： Supplementation of SI could reduce the formation of arteriosclerosis and protect the aorta and liver of rats fed on high lipid diet from damage. SI also had effects on the expression of PPARδ in rats fed on high lipid diet.