清肝化瘀方药对大鼠肝癌前病变中卵圆细胞表型的影响

Effect of A Traditional Medicine Oral Solution on Phenotypes of Hepatic Precancerous Lesion in Rats

背景与目的:探讨清肝化瘀方药阻断二乙基亚硝胺(Diethylnitrosamine,DEN)Solt_Farber二步法致大鼠肝癌前病变的机制。材料与方法:用DEN建立Solt_Farber二步法肝癌前病变短期动物模型,以不同浓度的清肝化瘀方药进行干预。透射电镜观察此模型中卵圆细胞的超微结构;酶组织化学方法检测γ_谷胺酰转肽酶(γ_GT酶)、葡萄糖_6_磷酸酶(G_6_P酶)的表达变化;免疫组织化学方法检测甲胎球蛋白(AFP)、白蛋白(ALB)表达的变化。结果:清肝化瘀方药能明显抑制肝癌前病变中肝细胞γ_GT酶的表达,增加G_6_P酶的表达;同时降低卵圆细胞及肝细胞中AFP的表达,增加ALB的表达。结论:清肝化瘀方药可有效阻断肝癌前病变,大剂量组较小剂量组作用明显,其作用机制可能与诱导卵圆细胞向肝细胞主向分化有关。

BACKGROUND ＆ AIM： To investigate solution on phenotypes of hepatic precancerous lesion model of prehepatocarcinoma in rat was established by the effect of a traditional medicine, ＂qing gan hua yu＂ oral in rats. MATERIALS AND METHODS ： A Solt-Farber two-step test diethylnitrosamine administration, to investigate the expression of γ-GT, G-6-P enzyme in different groups by enzyme-histochemisty, structure character of oval cell by electron microsocopy; the expression of AFP, ALB by immunohistochemistry. RESULTS： The concoction could prevent rat hepatic precancerous lesions by decreasing the expression of γ-GT and increasing the expression G-6-P enzyme. By electron microsocopy, we observed oval cells in model group were small with high nucleo-cytoplasmic ratio,while in treatment groups cells were larger and contained more rough endoplasmic reticulum. By immunohistochemistry, we found the expression of ALB was stronger in concoction group, although labeling for AFP was stronger in model group. CONCLUSION： The traditional medicine ＂qing gan hua yu＂ oral solution could prevent hepatic precancerous lesion rats,and was related to induction of rat hepatic oval cells to differentiate into mature hepatocytes.