摘要
背景与目的:探讨RUNX3基因表达与胃癌发生发展和转移的关系。材料与方法:采用羊抗人RUNX3蛋白抗体和枸橼酸_微波_SP免疫组织化学方法检测胃癌标本和正常胃组织阳性细胞数及阳性率,并采用Westernblot检测胃癌中RUNX3蛋白的表达状况。结果:免疫组化结果表明RUNX3在正常胃组织中阳性率100%,在胃癌中阳性率为51.13%,两者间的差异具有统计学意义(P<0.05);RUNX3蛋白的丢失在胃癌中占48.87%,并且与胃癌的组织类型、淋巴结转移状况有关。Westernblot结果表明RUNX3在胃癌组织中表达明显低于相应正常胃组织,且高分化胃癌中RUNX3蛋白表达高于低分化胃癌。结论:RUNX3可能是胃癌发生中重要的候选抑癌基因。
BACKGROUNG ＆ AIM： This study was designed to investigate the relationship between RUNX3 protein expression and gastric carcinogenesis and lymph node metastasis. MATERIALS AND METHODS： Detecting RUNX3 protein expression in gastric carcinoma by citrate-microwave-SP immunohistochemical method and westem blot. RESULTS： The positive rate of RUNX3 was 100% in normal gastric tissues and 51.13% in gastric carcinoma. 48.87% gastric carcinoma had loss of RUNX3 protein and correlated with histological types and lymph node metastasis. The expression of RUNX3 was obviously lower in gastric carcinoma than in normal stomach as shown by westem blot, RUNX3 protein in well differentiated was higher than in poorly differentiated gastric carcinoma. CONCLUSION： RUNX3 gene may be one of the important candidate tumor suppressor genes in carcinogenesis of C,C.
出处
《癌变．畸变．突变》
CAS
CSCD
2006年第6期455-458,共4页
Carcinogenesis,Teratogenesis & Mutagenesis
