人脑胶质瘤白介素13受体基因表达与肿瘤增殖活性的关系研究

Expression of interleukin-13 receptor and its relationship to proliferation activity of human gliomas

目的探讨人脑胶质瘤白介素13受体(IL-13R)基因表达与肿瘤增殖活性的关系。方法对6例正常脑组织,50例人脑胶质瘤和2个脑瘤体外细胞系采用RT-PCR法和免疫组织化学法检测IL-13R。结果人脑胶质瘤组织IL-13RαmRNA总阳性表达率70%,正常脑组织中仅1例有极弱的表达；2例恶性胶质瘤体外细胞系均高表达。IL-13RαmRNA表达率和表达丰度与胶质瘤分级(前者rs=0．87,P＜0．01；后者rs=0．69,P＜0．01)、肿瘤增殖活性Ki-67LI(r=0．64,P＜0．01)呈正相关,即胶质瘤恶性程度越高,IL-13RαmRNA表达率和表达水平越高。结论IL-13Rα基因在人脑胶质瘤中表达上升,与肿瘤的分级和肿瘤增殖活性呈正相关,可作为预测某些肿瘤治疗效果及监测复发的指标之一。

Objective To explore the gene expression of interleukin-13 receptor （IL-13R） α2 and its relationship to proliferation activity of human gliomas. Methods The gene expression of IL-13Rαin 50 human gliomas, 2 malignant human glioma cell lines and 6 normal brain tissues were studied by RT-PCR. Ki-67 labeling index （ Ki267 LI） of all sample were detecteded by immunohistochemical staining. Results Only one normal brain tissues expressed very low IL-13R α2 mRNA, whereas 35（70% ） of 50 human brain tumors expressed IL-13R α2 mRNA. The positive rate and expression level of IL-13R α2 mRNA were increased with the ascending of WHO tumor grade. （former： rs =0. 87; letter： rs =0. 69,P 〈0. 01 ）. The difference of positive rate and expression level of IL-13R 2α mRNA between the low grade and high grade tumors was statistically significant, the proliferation activity of gliomas evaluated by Ki-67LI （Ki-67 Labeling Index, Ki-67LI） was positively correlated with IL-13R α2 gene expression and the tumor grade. Conclusion In human cerebral gliomas, IL-13R α2 genes may play an role in the malignant progression. The expression level of malignancy in molecular level and selecting the target of gene therapy.