P33^(ING1b)在口腔鳞状细胞癌中的表达及其临床意义

Expression and clinical significance of P33^(ING1b) protein in oral squamous cell carcinoma

目的:观察P33ING1b蛋白在口腔鳞状细胞癌中的表达情况,探讨其临床病理意义。方法:采用免疫组织化学ABC法,检测20例正常口腔黏膜和57例口腔鳞状细胞癌石蜡存档标本中P33ING1b的表达情况,并分析P33ING1b蛋白表达情况与患者临床病理特征的关系。结果:P33ING1b在大部分正常黏膜有表达,以细胞核为主,阳性表达率为90.00%,在口腔鳞状细胞癌中的表达以细胞核、细胞质共同着色为主,阳性表达率为24.56%,二者比较差异有显著性(P<0.05)。口腔鳞状细胞癌中淋巴结转移组阳性表达率为46.15%(12/26),淋巴结无转移组阳性表达率为6.45%(2/31),二者比较有显著性差异(P<0.05)。结论:P33ING1b蛋白在口腔鳞状细胞癌的发生发展中可能有重要的作用。其表达由细胞核转移到细胞质可能是主要机制;P33ING1b蛋白的表达与淋巴结转移有关,可以作为判断预后的一个潜在生物学标志。

Objective：To study the expression and clinical significance of P33^ING1b protein in oral squamous cell carcinoma（OSCC）. Methods： ABC imnmnohistochemical method was used to investigate the expression of P33^ING1b protein in paraffin blocks of 20 cases of normal oral mucosa and 57 of OSCC. The relationship between P33^ING1b expression and clinical data of the patients was analyzed. Results：In normal oral mucosa P33^ING1b protein was observed mostly in cell nuclear, but in OSCC in cell nuclear and cytoplasm. P33^ING1b was expressed in 90.00% of the cases of normal mucosa and 24, 56% of OSCC（ P 〈 0.05 ）. The positive rate of P33^ING1b protein expression was 46. 15% （12/26） in lymph node metastasis group and 6.45% （2/31） in non-metastasis group（ P 〈 0.05）. The P33^ING1b protein expression was not correlated witb gender, age, as well as tumor location （P 〉 0.05）. Conclusions：The P33^ING1b may play an important role in the tumorgenesis and development of OSCC,one of the mechanism most probably is the translocation of the protein from nuclear to cytoplasm.