亚抗菌剂量的米诺环素对大鼠实验性牙周炎治疗作用的初步观察

Effects of minocycline at subantimicrobial dosage in the treatment of experimental periodontitis in rats

目的:初步观察亚抗菌剂量的米诺环素在大鼠实验性牙周炎中的治疗作用。方法:由24只成年雄性SD大鼠组成3个实验组:1组是模型组:仅诱导牙周炎;2组是治疗组:诱导牙周炎且用亚抗菌剂量的米诺环素治疗;3组是阴性对照组:假手术(仅腹腔麻醉)。牙周炎的诱导采用丝线结扎法并辅以100g/L蔗糖水为饮料。动物于实验的28d和58d处死,观察指标为:(1)视觉指标:牙松动度(MT),牙龈指数(GI)和牙槽骨丧失(ABL);(2)组织学指标:牙周组织中单核细胞的渗出数、破骨细胞数和牙周组织胶原的含量。资料采用方差分析统计学处理。结果:与模型组相比,28d和56d指标均显示亚抗菌剂量的米诺环素能显著抑制GI,MT,ABL和破骨细胞的形成,56d指标表明亚抗菌剂量的米诺环素能显著抑制牙周组织中单核细胞的渗出和胶原组织的降解。结论:亚抗菌剂量的米诺环素能有效抑制牙槽骨的吸收和牙周胶原纤维的降解,减缓大鼠实验性牙周炎的发展进程。

Objective： To evaluate the effectiveness of minocycline at subantimicrobial dosage in the treatment of experimentally induced periodontitis in rats. Methods： Periodontitis was induced by silk ligature technique on 4 second molars in each of 16 adult male Sprague-Dawley rats and the animals were fed with 100 g/L sucrose drink. The rats were divided into 2 groups： （ 1 ） periodontitis group without treatment ; （ 2 ） treatment group, the rats with periodontitis were treated by systemic administration of minocycline at 5 mg/（ kg · d）. Another 8 normal rats were used as the controls. Assessment was carried out at clay 28 and 56 using a number of different visual, histological and uhrastructure approaches. Visual assessment included tooth mobility （ TM ） , gingival index （ GI ） , and alveolar bone loss. Histological examination included monocyte effusion,resorption lacunae with osteoelasts and percentage of the periodontal collagen. The collected data were statistically analyzed using variance test. Results： Systemic administration of minocycline at subantimicrobial dosage can significantly reduce GI, TM, resorption lacunae with osteoclasts and alveolar bone loss either at day 28 or at clay 56 ; significantly inhibited monocyte effusion and the collagen degradation in the periodontium at day 56 in rats with periodontitis. Conclusion： Minocycline at subantimicrobial dosage may decrease alveolar bone loss and osteoclasts formation in periodontium with periodontitis.