NS-398对人舌鳞癌细胞系Tca8113表达VEGF的影响

Effects of NS-398 on vascular endothelial growth factor expression in human tongue squamous cell carcinoma Tca8113 cell line

目的:研究COX-2抑制剂NS-398对Tca8113细胞系表达血管内皮生长因子(VEGF)的影响。方法:应用逆转录聚合酶链反应(RT-PCR)及Westernblot方法,在环氧合酶-2抑制剂NS-398作用的基础上,观察NS398对Tca8113细胞系表达VEGF的影响。结果:NS-398可下调Tca8113细胞内VEGF的表达,且表现出一定的时间依赖性关系。结论:NS-398可能在COX-2参与口腔鳞癌新生血管生成的过程中起重要作用。

Objective：To study the effects of NS-398 on the expression of vascular endothelial growth factor （VEGF） in human tongue squamous cell carcinoma Tca8113 cells. Methods ：Tca 8113 cells were exposed to the COX-2 inhibitor NS-398 at 150 μmol/L for 48,72 and 96 h respectively, then cells were collected. VEGF expression was examined by RT-PCR and Western blot. Results：NS-398 inhibited VEGF mRNA and protein expression time-dependently in Tca8113 cells. Conclusion：NS-398 may inhibit VEGF expression in tongue squamous cell carcinoma.