X射线全身照射对小鼠脾细胞和腹腔巨噬细胞凋亡的影响

Effects of whole-body irradiation with X-rays on apoptosis in mouse splenocytes and peritoneal macrophages

目的:观察不同剂量X射线全身照射对小鼠脾细胞和腹腔巨噬细胞凋亡的影响。方法:Annexin-V和PI双染后,采用流式细胞术检测小鼠脾细胞和腹腔巨噬细胞在照射后不同时间的细胞凋亡变化。结果:2 GyX射线全身照射与假照射组比较,小鼠脾细胞凋亡百分率在照射后24 h明显升高(P<0.05),腹腔巨噬细胞凋亡百分率从照射后4 h开始明显升高(P<0.05,P<0.01),持续到48 h(P<0.001);0.075 Gy X射线全身照射与假照射组比较,小鼠脾细胞凋亡百分率在照射后24 h明显降低(P<0.05),腹腔巨噬细胞凋亡百分率从照射后2 h开始至照射后16 h均有显著降低(P<0.05)。结论:较高剂量辐射促进小鼠脾细胞和腹腔巨噬细胞凋亡,而低剂量辐射对小鼠脾细胞和腹腔巨噬细胞凋亡没有促进作用,并且能降低免疫细胞凋亡。

Objective To study the effects of whole-body irradiation （WBI） with different doses of X-rays on apoptosis in mouse splenocytes and peritoneal macrophages. Methods The apoptosis percentages of mouse splenocytes and peritoneal macrophages were detected with flow cytometry （FCM） at different time after the whole-body X-irradiation using the staining of Annexin-V and PI. Results As compared with the control, the percentage of apoptosis in mouse splenocytes began to increase gradually 24 h after WBI with 2 Gy X-rays （P〈 0.05）, and the percentage of apoptosis in mouse peritoneal macrophages arose 4 h after 2 Gy irradiation, and kept higher level till 48 h （P〈0.05, P〈0.01, P〈0.001）. After WBI with 0.075 Gy X-rays, the percentage of apoptosis in mouse splenocytes reduced at 24 h （P 〈 0. 05）, and the percentage of apoptosis in peritoneal macrophages significantly reduced from 2 h to 16 h （P〈0.05） as compared with the control. Conclusion High dose irradiation can stimulate apoptosis in mouse splenocytes and peritoneal macrophages, while low dose irradiation can cause an opposite effect and even suppress immunocyte apoptosis.