长春地区乙型肝炎病毒基因分型

Genotypes of hepatitis B virus in Changchun

目的:探讨长春地区慢性乙型肝病患者中乙型肝炎病毒(HBV)基因型特点。方法:慢性乙型肝病患者血清中提取HBV DNA,经巢式聚合酶链法(nested-PCR)扩增HBV S基因区片断,利用双脱氧链末端终止法检测HBV S区核苷酸序列并进行基因型分析。结果:39株HBV中C基因型23株,其中16株为C基因型adr亚型,7株为C基因型adw亚型;16株为B基因型adw亚型;未发现ayr及ayw型;有2株存在“a”决定簇变异;与参照序列比较,S基因存在多处点突变,部分点突变导致所编码的氨基酸变异;同以往研究的结果类似,未发现癌特异性的HBV S基因变异。结论:研究对象中HBV存在C基因型和B基因型,C基因型为主要基因型;HBV相关性慢性肝病患者中HBV S基因存在序列多态性。

Objective To investigate the genotypes of hepatitis B virus in chronic liver disease patients in Changchun, China . Methods HBV DNA extracted from the 39 serum samples of chronic liver disease patients were amplified by nested polymerase chain reaction （nested-PCR） to obtain the S gene sequences, and all the PCR products were sequenced by dideoxy-chain-termination method. Results The S gene nucleotide sequence was acquired and the corresponding amino acid sequence was deduced. Compared with the consensus sequence, 16 strains belonged to genotype C/adr and 7 strains to genotype C/adw; 16 strains belonged to genotype B/adw; and the amino acids of antigen determinant ＂a＂ of two strains changed in the 2 serum samples. There were many mutational positions and some of these caused amino acid residue substitution. No special point mutation was found which was similarly related to hepatocellular carcinoma. Conclusion In chronic liver diseases patients infected with HBV , S gene sequence of HBV has its variability.