MMP-2和TIMP-2在肺癌组织中的表达及其与微血管密度的关系

Expressions of MMP-2 and TIMP-2 in lung cancer tissue and their relationships with microvessel density

目的:研究基质金属蛋白酶-2(MMP-2)和组织基质金属蛋白酶抑制剂-2(TIMP-2)在人肺癌组织中的表达及其与微血管密度(MVD)的关系。方法:免疫组织化学方法检测90例肺癌组织和40例癌周组织中MMP-2和TIMP-2的表达,Ⅷ因子染色计数局部MVD,分析MMP-2和TIMP-2与MVD的关系。结果:免疫组织化学染色结果经IPP软件分析显示,肺癌组织MMP-2的表达值高于癌周组织(P<0.001),腺癌高于鳞癌(P<0.05),小细胞癌最高(P<0.05),并发淋巴结转移的肺癌组织高于无转移组(P<0.001);肺癌组织TIMP-2的表达低于癌周肺组织(P<0.001),在各种不同病理类型肺癌中的表达差异无显著性(P>0.05),并发淋巴结转移的肺癌组织表达较低(P<0.05)。MMP-2表达强度随着MVD的增高而增强,TIMP-2表达程度随着MVD的增高而减弱(P<0.05)。结论:MMP-2在各种不同病理类型的肺癌组织中有不同程度的表达,MMP-2和TIMP-2的表达水平与肺癌局部的MVD有关。

Objective To study the expressions of metalloproteinase 2 （MMP-2） and tissue inhibitors of metalloproteinase 2 （TIMP-2） in lung cancer, and their relationships with microvessel density （MVD）. Methods The expressions of MMP-2 , TIMP-2 and Ⅷ factor were tested by immunohistochemical staining in 90 cases of lung cancer tissue and 40 cases of para-cancer lung tissue. Results The immunohistochenmical staining results analyzed by IPP software indicated that MMP-2 and TIMP-2 expressed in all pathological types of lung cancer. The expreesion level of MMP-2 in lung cancer tissue was higher than that in para-cancer tissue （P〈 0. 001）, it was higher in adenocarcinoma than that in squamous carcinoma （P〈0.05）, and was the highest in SCLC （P〈0.01）; the expresion level in lung cancer tissue with lymph node metastasis was higher than that in lung cancer tisse without lymph node metastasis （P〈0.001）. The expression level of TIMP-2 in lung cancer tissue was lower than that in para-cancer tissue （P 〈 0. 001）, there was no significant difference between various pathological types of lung cancer （P〈0.05） ; the expression level of TIMP-2 in lung cancer with lymph node metastasis was lower （P〈 0.05） . The expression level of MMP-2 increased and TIMP-2 decreased with the increasing of MVD. Conclusion Expressions of MMP-2 in differet tissue are on different levels. The expressions of MMP-2 and TIMP-2 protein are closely correlated with the MVD of lung cancer.