阴道加德纳菌四环素耐药基因tetM的体外扩增及其产物的序列测定

The amplification and nucleotide sequences analysis of the tet M genes from tetracycline resistance Gardnerella vaginalis

利用聚合酶链式反应（ＰＣＲ）对５株耐四环素阴道加德纳菌（Ｇａｒｄｎｅｒｅｌａｖａｇｉｎａｌｉｓ）（ＭＩＣ＞１６ｍｇ／Ｌ）的耐药基因ｔｅｔＭ进行体外扩增。核苷酸序列测定证实，１株耐四环素阴道加德纳菌的ｔｅｔＭ核苷酸序列与另４株ｔｅｔＭ的序列不同，两者的同源性为９８．４％，同时又和Ｔｎ１５４５、Ｔｎ９１６部分同源。说明在阴道加德纳菌中ｔｅｔＭ基因存在多态性，其获得方式、来源途径均不相同。

In this study, 5 clinical isolates of Gardnerella vaginalis were screened for high level tetracycline resistance (MIC>16mg/L) and for the presence of the tetracycline resistances gene tet M. The nucleotide sequence of the tet M, located on the chromosome of G.vaginalis has been amplified by polymerase chain reaction (PCR) and then determined. Result showed that the nucleotide sequence of the tet M genes of one G.vaginalis strain has some difference from that of the other four strains. These data suggest that these tet M genes may evolved and acquired the resistance determinant from different sources.