高效液相色谱法测定大米38中抑虫肼的残留量

Determination of tebufenozide in rice by high performance liquid chromatography

建立了高效液相色谱法测定大米中抑虫肼残留量的方法。样品采用丙酮-水提取、二氯甲烷萃取、硅胶和中性氧化铝柱净化后，用Nucleosil 100—5 C18（250mm×4．6mmi．d，，5μm）分离，以乙腈-水（体积比55：45）作流动相，流速1．0mL·min^-1，于234nm波长处检测。结果表明，样品的加标平均回收率为93．5％～81．2％，相对标准偏差为2．03％-5．50％，样品测定低限（LOQ）为0．025mg·kg^-1。该方法具有简便、快捷、灵敏、重珊陛好。

A high performance liquid chromatographic method （HPLC） was developed for the determination of tebufenozide residue in rice. The tebufenozide residue in the sample was extracted with a solution of acetone - water. The supernatant liquid was evaporated , and reextracted with dichlormethane and cleaned up with acetone - n - hexane （ 1 ： 1 ） on a silica gel and aluminium column. The chromatographic separation was performed on a Nucleosil 100- 5 C18 column（250 mm ×4.6 mm i. d. ,5μm）using acetonitrile -water（55：45 ,v/v） as moble phase at a flow rate of 1.0mL/min and UV detector at 234nm. The results showed that the average recoveries were 93. 40% -85.22% and the relative standard deviations were 2.03% - 5.50%. The method is simple, rapid, sensitive and reproducible.