当归干预肺间质纤维化的作用途径研究

Study to Inhibitive Effect of Angelica Sinensis on the Development of Pulmonary Fibrosis

目的:研究当归抗肺间质纤维化形成的作用机制,并与激素在疗效和作用环节方面进行比较。方法:大鼠随机分为空白对照组、肺纤维化模型组、氢化可的松治疗组和当归治疗组。气管内注入博莱霉素造成动物模型后,于当天开始每天给药,分别于7、28天处死,取肺组织进行HE染色、MASSON染色,取血测血流变、内皮素-1、血管紧张素Ⅱ、血栓素。结果:与模型组比较两治疗组肺纤维化程度明显减轻,且组间无差异;内皮素-1、血管紧张素Ⅱ、TXB2、血流变指标当归组明显低于模型组和激素组,激素组则高于模型组。结论:当归具有保护肺血管内皮细胞的作用,从而抑制博莱霉素引起的肺纤维化形成,同时又可改善血流变,避免激素使用时引起的血液黏度增高,尚可通过抑制炎症介质TXB2抑制肺损伤,作用环节与激素不同。

Objective： To observe the inhibitive effect of angelica sinensis on the development of pulmonary fibrosis. Methods： Rats were divided into four groups at random： normal control group,untreated animal group, angelica sinensis treated group and hydrocortisone treated group. Fibrosis was induced in rats by intratracheal instillation of bleomycin , then the rats were received daily stomach instillation. On days 7and 28 ,six rats in each group were killed and lungs and serum were harvested for hisopathologlcal studies ,and detection for ET - 1 ,TXB2, and Ang Ⅱ. Results ： The degree of fibrosis decreased significantly in the two treated groups on day 7and 28 . Conclusion： Inhibitive effect of angelia sinensis on lung is equal to hydrocortisone. Angelia sinensis also improve the value of hemorheology and inhibit vasoactive material which play an important role in the process of pulmonary fibrosis induced by bleomyein.