WSSV感染中国对虾肌肉与鳃膜蛋白变化及差异蛋白的鉴定

Changes of protein profile and identification of differentially expressed proteins in gills and muscles of Fenneropenaeus chinensis infected by white spot syndrome virus

采用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）、基质辅助激光解吸电离飞行时间质谱分析（MALDI-TOF／MS）、生物信息学技术，研究了WSSV感染中国对虾后鳃膜蛋白和肌肉组织蛋白的变化。结果表明，WSSV感染中国对虾12h后，鳃膜蛋白27kD蛋白增强，至72h，27kD蛋白增强显著。肌肉组织蛋白在感染48h后，出现1条明显差异分子量为22kD的蛋白带。通过对该差异蛋白进行MALDI-TOF／MS的肽质量指纹图谱及其生物信息分析，鉴定为钙离子结合蛋白，其在对虾抗病毒感染免疫中的功能值得关注。

Sodium dodecyl sulfate-polyacrylamide gel electrophoresis （SDS-PAGE）map, matrix assisted laser desorption ionization time of flight mass spectrometry （MALDI-TOF-MS） and bioinformatics were used to analysis and identify differential proteins in the gills and muscles of F. Chinensis infected by white spot syndrome virus（WSSV）. The results showed that 27kD protein in gills was intensified after 12h infection and this band increased significantly after 72h infection. There exited one new band of 22kD protein in SDS-PAGE map in the muscles after 48h infection. It was identified as a calcium-binding protein（alpha-b-and-A chains） by MALDI-TOF/ MS and bioinformatics analysis.