茵陈五苓散对动脉粥样硬化大鼠PDGF-A mRNA表达的影响

The Effect of Yinchenwuling Powder(茵陈五苓散) on the Expression of PDGF-A mRNA in Atherosclerosis Rats

目的观察茵陈五苓散(YCL)对动脉粥样硬化(AS)大鼠血管平滑肌细胞(VSMC)PDGF-AmRNA表达的影响。方法70只雄性Wistar大鼠随机分为正常组、模型组、YCL组、Met组,采用高脂饲料喂饲法复制大鼠AS模型,造模1月后抽样检测大鼠主动脉,以发现AS斑块为造模成功指标。治疗1个月后分别检测血脂、血液流变学,用HE染色观察主动脉组织学变化,用透射电镜观察大鼠主动脉组织超微结构变化,用PT-PCR技术检测大鼠VSMC基因PDGF-AmRNA的表达。结果YCL组与Met组比较,TC、TG、LDL的降低和HDL的升高均显著,其降低血浆黏度、全血(中切)黏度、红细胞压积、血小板黏附率明显,可维持主动脉组织结构,下调基因PDGF-AmRNA的表达。结论茵陈五苓散具有良好的抗AS作用,其调整相关基因PDGF-AmRNA的表达可能是其治疗的分子机理。

Objective:To observe the effect of Yinchenwuling Powder(茵陈五苓散) atherosclerosis(AS) rats.Methods:70 wistar rats were divided into groups:the normal,the model,the control and the therapy group.The AS model of rats was made by feeding with high grease food and AS plague was observed in the aorta after 1 mouth of feeding,Yinchenwuling Powder was used in the group of therapy;tablet Gypenosides was used in the group of control.The level of serum lipid,blood sugar and insulin were tested after 1 month;the change of aorta histology was observed by HE staining;the ultrastructure of aorta tissue was observed by transmission electronic microscope;the apoptosis of aorta vascular smooth muscle(VSMC) and the expression of action were evaluated by immunocytochemistry;the expression of VSC genes such as PDGF-A mRNA were tested by RT-PCr.Results:In the therapy group,the levels of TC,TG,plasmviscosity,erythrocyte hematocrit and platelet adhesion ratio decreased significantly;the level of HDL,insulin sensitivity index increased;the plague regressed;the histology of aorto didn't change much;the expression of action decreased;the gene expression of PDGF-A were downregulated and p53 was upregulated.Compared with the model group,the expression of PDGF-A mRNA was downregulated in the therapy group.Conclusion:Yinchenwuling Powder has significant effect on AS and the therapeutic molecular mechanism is inhibition of apoptosis and adjustment of the correlated gene expression.