不同方法制备的海星皂甙细胞毒性评价

Cell Toxicity of Two Saponins Extracted by Different Methods from Starfish ( Asterias amurensis)

考察超临界CO2萃取、乙醇萃取两种方法制备的南海产多棘海星皂甙的溶血过程,采用分光光度法测定溶血指数,结合红细胞形态血观察,评价其细胞毒性。结果显示,两种方法制备的海星皂甙都具有明显溶血作用,超临界CO2萃取、溶剂萃取的海星皂甙溶血指数分别为19231,12821,超临界CO2萃取的海星皂甙保持了更强的细胞毒性,这为海星皂甙制备工艺的选择,探索其物质基础提供了参考依据。

According to the hemolytic process, the cell toxicity of two saponins extracted by supercritical CO2 and organic solvent from Asterias amurensis in the South China sea was evaluated. The result showed all the saponins prepared by different methods had hemolysis to the red blood cell. Hemolytic activity showed that these saponins destroyed the membrane of the red blood cell The hemolytic index of two saponins extracted by supercritical CO2 and organic solvent were 19231 and 12821 respectively. The result provides reference for the selection extraction technique and material basis for toxicity.