摘要
目的 建立应用自动浊度微生物分析仪定量测定多种维生素营养片剂中生物素含量的方法。方法 应用自动浊度微生物分析仪对样品进行自动稀释，自动检测标准溶液和样品溶液中的菌体生长浊度，通过测定细菌生长和繁殖强度间接地测定出样品中的生物素含量：结果方法的线性范围0．05～0．9ng／ml，曲线相关系数r为0．9972，精密度为3，75％，回收率平均值为102．37％。结论 方法操作简单、灵敏度高、处理量大，适合生产企业的批量质量控制和检测。
Established an automated microbiological assay, which is for determination the d-biotin content in multi-vitamin tablets. In this assay, the Autoturb^3 system was used for measuring the turbidity of bacteria＇ s growth. It was found that the range of linearity is 0.05 -0. 9 ng/ml, correlation coefficient of calibration curve is 0. 9972, precision is 3.75% , and recovery rate is 102.37%. The available data show the assay to be a rapid, accurate method and suitable for easily handling a large numbr of samples in manufacturing enterprises.
出处
《广州医药》
2006年第6期58-60,共3页
Guangzhou Medical Journal
